B-chromosome ribosomal DNA is functional in the grasshopper Eyprepocnemis plorans.

PloS One
Mercedes Ruiz-EstévezJ P M Camacho

Abstract

B-chromosomes are frequently argued to be genetically inert elements, but activity for some particular genes has been reported, especially for ribosomal RNA (rRNA) genes whose expression can easily be detected at the cytological level by the visualization of their phenotypic expression, i.e., the nucleolus. The B(24) chromosome in the grasshopper Eyprepocnemis plorans frequently shows a nucleolus attached to it during meiotic prophase I. Here we show the presence of rRNA transcripts that unequivocally came from the B(24) chromosome. To detect these transcripts, we designed primers specifically anchoring at the ITS-2 region, so that the reverse primer was complementary to the B chromosome DNA sequence including a differential adenine insertion being absent in the ITS2 of A chromosomes. PCR analysis carried out on genomic DNA showed amplification in B-carrying males but not in B-lacking ones. PCR analyses performed on complementary DNA showed amplification in about half of B-carrying males. Joint cytological and molecular analysis performed on 34 B-carrying males showed a close correspondence between the presence of B-specific transcripts and of nucleoli attached to the B chromosome. In addition, the molecular analysis revealed a...Continue Reading

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Citations

Aug 6, 2013·Cellular and Molecular Life Sciences : CMLS·Andreas HoubenJeremy N Timmis
Jan 9, 2014·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Mercedes Ruiz-EstévezMaría Dolores López-León
Jul 6, 2014·Molecular Genetics and Genomics : MGG·Mercedes Ruiz-EstévezJuan Pedro M Camacho
Aug 7, 2013·BMC Biology·Vladimir A TrifonovAlexander S Graphodatsky
Sep 7, 2013·Genetica·Mercedes Ruíz-EstévezJuan Pedro M Camacho
Dec 10, 2015·Proceedings. Biological Sciences·Katarzyna MichalakPawel Michalak
Dec 30, 2014·Molecular Cytogenetics·Alexey I MakuninVladimir A Trifonov
Jan 12, 2016·Comparative Cytogenetics·Maria L TerencioEliana Feldberg
Sep 17, 2014·PloS One·José Carlos Pansonato-AlvesFausto Foresti
Apr 4, 2017·Scientific Reports·Beatriz Navarro-DomínguezJuan Pedro M Camacho
Sep 21, 2017·Genes·Manuel A Garrido-Ramos
Feb 15, 2018·Genes·Radka Symonová, W Mike Howell
Dec 17, 2017·Scientific Reports·Beatriz Navarro-DomínguezMaría Dolores López-León
Sep 27, 2014·Molecular Genetics and Genomics : MGG·Kuo-Wei KaoYa-Ming Cheng
Oct 27, 2018·Genes·André MarquesAndreas Houben
Aug 31, 2017·BMC Genetics·Diogo MilaniDiogo Cavalcanti Cabral-de-Mello
Dec 20, 2018·Genes·Frances E ClarkThomas D Kocher
Apr 11, 2018·Cytogenetic and Genome Research·Geovana C MalimpensaOrlando Moreira-Filho

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Methods Mentioned

BETA
PCR
acetylation
electrophoresis

Software Mentioned

BLAST ( Basic Local Alignment Search
Bioedit
Torrox

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