B-CLL cells express true endogenous E receptor after culture with T-cells and mitogens

British Journal of Haematology
C P Worman, J C Cawley

Abstract

Culture studies of peripheral blood mononuclear cells from seven typical cases of B-cell chronic lymphatic leukaemia (CLL) showed that, in the presence of PHA or Con A and greater than or equal to 20% autologous or allogeneic T-cells, CLL cells express true sheep erythrocyte (E) receptor after 2-5 d, but fail to stain with a panel of anti-T-cell sera. In contrast to similarly cultured hairy cells (HCs), the cultured CLL cells continue to express light-chain-restricted surface immunoglobulin (SIg) and show no change in their morphology. The continued SIg positivity and staining with a panel of monoclonal antibodies indicated that the high proportion of E+ cells could not be attributed to the outgrowth of normal T-cells or to mitogen-induced non-specific stickiness. Repeated addition of supernatants from cultured T-cells caused no phenotypic changes and therefore excluded the possibility of shedding and passive acquisition of E receptor. The points of similarity and difference between the responses of CLL cells and HCs cultured in a like manner are briefly considered in relation to the possible lineages of these two cell types.

References

Jan 1, 1978·Journal of Immunological Methods·C P Worman
Dec 1, 1978·British Journal of Haematology·A K SinghG Wetherley-Mein
Jul 21, 1977·Nature·G F BurnsJ C Cawley
Jan 1, 1975·Clinical Immunology and Immunopathology·C C HsuR C Williams
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Jan 1, 1981·Leukemia Research·M F GreavesG Goldstein
Apr 1, 1981·Immunology Today·E L Reinherz, S F Schlossman

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Citations

May 1, 1992·British Journal of Haematology·A S KurecF R Davey
Jan 1, 1986·Journal of Immunopharmacology·A P EfremidisJ G Bekesi
Feb 1, 1984·The American Journal of Medicine·R R TubbsJ K Weick
Jan 1, 1991·Leukemia Research·P G BriggsR C Atkins
Oct 1, 1985·The British Journal of Dermatology·R GreenwoodI Lauder

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