PMID: 8972854Dec 1, 1996Paper

B-lineage regulated polyadenylation occurs on weak poly(A) sites regardless of sequence composition at the cleavage and downstream regions

Nucleic Acids Research
S A MatisChristine Milcarek

Abstract

Early/memory and plasma B-cell lines and fibroblasts were analyzed for their ability to use a 5' proximal (variant) versus a 3' distal (constant) poly(A) site, in the absence of a competing splice, from a set of related constructs. The proximal:distal poly(A) site use (P:D ratio) of the resulting cytoplasmic poly(A)+ mRNA is a measure of poly(A) site strength. In this context the immunoglobulin gamma2b secretory-specific poly(A) site showed a P:D ratio of 1:1 in plasma cells, 0.43:1 in early/memory B-cells and an intermediate value in fibroblasts. Meanwhile, a construct with a proximal SV40 early-like poly(A) site produced mRNA with a P:D ratio of >50:1 in all cell types. Alterations in the region downstream of the proximal poly(A) addition site and at the site itself resulted in changes in the P:D ratio. However, these poly(A) sites, all with a P:D ratio of < or = 5:1, were used most efficiently in plasma cells. Constructs totally devoid of immunoglobulin sequences, but containing heterologous poly(A) sites producing mRNA with P:D ratios of < or = 5:1, were also used more efficiently in plasma cells. We therefore conclude that weak poly(A) sites, regardless of sequence composition, are used more efficiently in plasma cells tha...Continue Reading

References

Jan 1, 1991·Somatic Cell and Molecular Genetics·C GenoveseC Milcarek
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V T OiP Berg
Jan 15, 1996·Genes & Development·H LouS M Berget

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Citations

Jul 1, 1997·Nucleic Acids Research·G Edwalds-GilbertC Milcarek
Sep 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Micah J BensonAnjana Rao
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K MartincicC Milcarek
Feb 27, 2008·The Plant Journal : for Cell and Molecular Biology·Denghui XingQingshun Quinn Li
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Martha L Peterson
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J H Coyle, D A Lebman

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