B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3

International Journal of Molecular Sciences
Xiaoyan FanYouquan Bu

Abstract

B-Myb has been shown to play an important oncogenic role in several types of human cancers, including non-small-cell lung cancer (NSCLC). We previously found that B-Myb is aberrantly upregulated in NSCLC, and overexpression of B-Myb can significantly promote NSCLC cell growth and motility. In the present study, we have further investigated the therapeutic potential of B-Myb in NSCLC. Kaplan⁻Meier and Cox proportional hazards analysis indicated that high expression of B-Myb is significantly associated with poor prognosis in NSCLC patients. A loss-of-function study demonstrated that depletion of B-Myb resulted in significant inhibition of cell growth and delayed cell cycle progression in NSCLC cells. Notably, B-Myb depletion also decreased NSCLC cell migration and invasion ability as well as colony-forming ability. Moreover, an in vivo study demonstrated that B-Myb depletion caused significant inhibition of tumor growth in a NSCLC xenograft nude mouse model. A molecular mechanistic study by RNA-seq analysis revealed that B-Myb depletion led to deregulation of various downstream genes, including insulin-like growth factor binding protein 3 (IGFBP3). Overexpression of IGFBP3 suppressed the B-Myb-induced proliferation and migration,...Continue Reading

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S GoldsteinR C Baxter
Jul 28, 1995·Gene·E W LamR J Watson
Apr 14, 1995·The Journal of Biological Chemistry·G RaschellàB Calabretta
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·A SalaC Peschle
Jan 15, 1998·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·S AnsieauA Leutz
Feb 12, 2002·The Journal of Biological Chemistry·Steven R SchwarzeDavid F Jarrard
Dec 6, 2002·Endocrine Reviews·Sue M Firth, Robert C Baxter
Jan 31, 2003·Molecular Carcinogenesis·Amir A JazaeriEdison T Liu
Nov 20, 2003·Cellular and Molecular Life Sciences : CMLS·M Joaquin, R J Watson
Oct 4, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Arturo Sala
Oct 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Munenori TakaokaHiroshi Nakagawa
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christophe GinestierFrançois Bertucci
Jul 16, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tomoaki NakajimaTakeshi Okanoue
Apr 12, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Diego F CalvisiRosa M Pascale
Aug 11, 2011·Cancer Research·Ivan Martinez, Daniel Dimaio
Dec 12, 2012·Experimental and Therapeutic Medicine·Qiang LvXichen Zhang
Feb 25, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M SinnC Denkert
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael E FeiginSenthil K Muthuswamy
Mar 29, 2014·Carcinogenesis·Ganji Purnachandra NagarajuSajani S Lakka
Jun 21, 2014·Current Pharmaceutical Design·Liling Tang, Jianguo Feng
Jul 2, 2014·Aging Cell·Sophia N MowlaParmjit S Jat
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Apr 6, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hsiu-Hua WangHuei Lee
May 31, 2017·International Journal of Molecular Sciences·Yuelei JinYouquan Bu
Aug 19, 2017·Biochemical and Biophysical Research Communications·Yelong ChenZhaoyong Liu

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Citations

Sep 21, 2020·Journal of Cancer Research and Clinical Oncology·M NientiedtM C Kriegmair

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Methods Mentioned

BETA
RNA-seq
transfection
protein assay
xenograft

Software Mentioned

Photoshop
SPSS16

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