B-Raf inhibition in conjunctival melanoma cell lines with PLX 4720

The British Journal of Ophthalmology
Aline I RiechardtEnken Gundlach

Abstract

Mutations in the gene coding for the kinase B-Raf are associated with tumour growth in conjunctival melanoma. The purpose of this study is to explore effects of pharmacological B-Raf inhibition in conjunctival melanoma cell lines. The B-Raf genotypes were assessed by PCR and subsequent sequencing. Cytotoxicity, cell viability, proliferation, apoptosis rate and phosphorylation rate of ERK and Akt were analysed in three different conjunctival melanoma cell lines under the influence of the B-Raf inhibitor PLX 4720 at various concentrations. The cell lines CRMM-1 and CM2005.1 showed the B-Raf V600E mutation, whereas CRMM-2 expressed a B-Raf wild type. CM2005.1 was highly sensitive to PLX 4720, showing a complete cytotoxic effect for >1 µM, as well as a significant concentration-dependent reduction of the proliferation rate and viability rate. Even though CRMM-1 also carries the B-Raf V600E mutation, it did not react as sensitive to PLX 4720 inhibition as CM2005.1, but showed a significant concentration-dependent reduction regarding proliferation and viability. PLX 4720 had only slight impact on CRMM-2 in high concentrations (10 µM) regarding cytotoxicity, proliferation and viability. Fluorescence-activated cell sorting analysis rev...Continue Reading

Citations

Jun 13, 2018·The Journal of Craniofacial Surgery·Fanglin HeRenbin Jia
Jun 28, 2019·Investigative Ophthalmology & Visual Science·Ikram El ZaouiAlexandre P Moulin
Nov 29, 2016·Ophthalmic Plastic and Reconstructive Surgery·Lora R Dagi GlassSuzanne K Freitag
Oct 4, 2017·Pigment Cell & Melanoma Research·Fiona P BaileyPatrick A Eyers
Apr 3, 2019·International Journal of Molecular Sciences·Paola SavoiaOttavio Cremona
Oct 30, 2020·Clinical Ophthalmology·Anastasia Gkiala, Sotiria Palioura
Sep 28, 2020·Survey of Ophthalmology·Yue ZengFang Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Henrike WestekemperRalf A Hilger
Expert Opinion on Investigational Drugs
Elisabeth LivingstoneDirk Schadendorf
The New England Journal of Medicine
Hiroyuki Morita, Ryozo Nagai
© 2021 Meta ULC. All rights reserved