PMID: 9185716Jun 11, 1997Paper

B7-1-transfected tumor vaccine counteracts chemotherapy-induced immunosuppression and prolongs the survival of rats bearing highly metastatic osteosarcoma cells

International Journal of Cancer. Journal International Du Cancer
M HayakawaT Uede

Abstract

To investigate the therapeutic efficacy of B7-1-expressing tumor vaccine on metastatic osteosarcoma, we introduced mouse B7-1 cDNA into a rat osteosarcoma cell line, MSK-8G. Flow cytometric analysis confirmed that the transfectants designated as B7-1-8G 10-1 and B7-1-8G 15-5 stably expressed B7-1 molecules on the cell surface. B7-1 transfectants were not only rejected by immunocompetent F344 rats but also conferred systemic immunity that protected against challenge with B7-negative parental osteosarcoma cells. In contrast, T-cell-deficient nude rats failed to reject B7-1 transfectants, indicating that T cells play a major role in the development of systemic immunity. We then conducted experimental therapies using irradiated B7-1-transfected tumor vaccine and methotrexate in an orthotopic implantation model. B7-1-transfected tumor vaccine significantly reduced the number of pulmonary metastatic nodules. Moreover, the combination of methotrexate and tumor vaccine further decreased the number of pulmonary metastatic nodules. Most important, the combined therapy with methotrexate and tumor vaccine resulted in a tumor-free condition as judged by the histopathological absence of tumors and survival of rats for more than 180 days. Fur...Continue Reading

References

Oct 1, 1993·Immunology Today·L ChenK E Hellström
Apr 15, 1994·Biochemical and Biophysical Research Communications·M InobeT Uede
Mar 1, 1995·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·N MitsuhashiH Niibe
Nov 4, 1993·Nature·M AzumaC Somoza
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MurakamiT Uede

❮ Previous
Next ❯

Citations

Aug 10, 2005·Journal of Translational Medicine·Satoshi KawaguchiToshihiko Yamashita
Dec 1, 2009·Neurosurgery Clinics of North America·James L FrazierAlessandro Olivi
May 8, 2001·Cancer Investigation·W S Ferguson, A M Goorin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.