Apr 18, 2015

Babela massiliensis, a representative of a widespread bacterial phylum with unusual adaptations to parasitism in amoebae

Biology Direct
I PagnierBernard La Scola

Abstract

Only a small fraction of bacteria and archaea that are identifiable by metagenomics can be grown on standard media. Recent efforts on deep metagenomics sequencing, single-cell genomics and the use of specialized culture conditions (culturomics) increasingly yield novel microbes some of which represent previously uncharacterized phyla and possess unusual biological traits. We report isolation and genome analysis of Babela massiliensis, an obligate intracellular parasite of Acanthamoeba castellanii. B. massiliensis shows an unusual, fission mode of cell multiplication whereby large, polymorphic bodies accumulate in the cytoplasm of infected amoeba and then split into mature bacterial cells. This unique mechanism of cell division is associated with a deep degradation of the cell division machinery and delayed expression of the ftsZ gene. The genome of B. massiliensis consists of a circular chromosome approximately 1.12 megabase in size that encodes, 981 predicted proteins, 38 tRNAs and one typical rRNA operon. Phylogenetic analysis shows that B. massiliensis belongs to the putative bacterial phylum TM6 that so far was represented by the draft genome of the JCVI TM6SC1 bacterium obtained by single cell genomics and numerous environ...Continue Reading

  • References67
  • Citations11

Mentioned in this Paper

Gene Polymorphism
Size
Ring Chromosomes
Lysinibacillus massiliensis
Genome
Genes
rRNA Operon
Ubiquitin
Isolation Aspects
Acclimatization

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