Babesiosis caused by Babesia vogeli in dogs from Uberlândia State of Minas Gerais, Brazil

Parasitology Research
Camila Oliveira Silva BarbosaGad Baneth

Abstract

Babesia is tick-transmitted protozoan parasites that infect mammalian hosts and have a major impact on farm and pet health-associated costs worldwide. This study aimed to test the prevalence of Babesia spp. infection in a small cohort of dogs at a veterinary hospital and to perform molecular characterization of the Babesia species causing the infection. For the PCR assay, 5 mL of blood was collected by venipuncture of the cephalic or radial veins in 300 dogs of different ages, sex, and breeds, which were presented to the veterinary hospital of the Federal University of Uberlândia between March 2015 and April 2016. In addition, a drop of blood was collected from the marginal blood vessels of the ear of dogs included in this study. Ninety-two (30.67%) were positive for Babesia spp., as determined by microscopic observation of the blood smear, revealing the presence of intra-erythrocyte merozoites. For molecular characterization by PCR, 17 samples were chosen from dogs who were tested positive for Babesia spp. by blood smears. Among them, B. vogeli was found to infect all 17 dogs, as determined by 99-100% sequence identity (closest GenBank match KT246307) using primers PIRO A/PIRO B. Our results indicate that the species observed ...Continue Reading

References

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Citations

Aug 7, 2020·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Fernanda Barbosa Dos SantosAlynne da Silva Barbosa
Jun 11, 2021·Parasitology Research·Filipe Dantas-TorresGertraut Altreuther

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Datasets Mentioned

BETA
KT246307
MN389563
MN389564

Methods Mentioned

BETA
PCR

Software Mentioned

Chromas Pro
BLAST

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