Bach2 is involved in neuronal differentiation of N1E-115 neuroblastoma cells

Experimental Cell Research
Ki Shuk ShimMarkus Hengstschläger

Abstract

Bach1 and Bach2 are evolutionarily related members of the BTB-basic region leucine zipper transcription factor family. We found that Bach2 downregulates cell proliferation of N1E-115 cells and negatively affects their potential to differentiate. Nuclear localization of the cyclin-dependent kinase inhibitor p21 is known to arrest cell cycle progression, and cytoplasmic p21 has been shown to promote neuronal differentiation of N1E-115 cells. We found that ectopic Bach2 causes upregulation of p21 expression in the nucleus and in the cytoplasm in undifferentiated N1E-115 cells. In differentiated cells, Bach2 specifically triggers upregulation of cytoplasmic p21. Our data suggest that Bach2 expression could represent a switch during the process of neuronal differentiation. Bach2 is not expressed in neuronal precursor cells. It would have negative effects on proliferation and differentiation of these cells. In differentiated neuronal cells Bach2 expression is upregulated, which could allow Bach2 to function as a gatekeeper of the differentiated status.

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y KimhiU Z Littauer
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·K IgarashiM Yamamoto
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Y ChanY W Kan
Feb 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·H MotohashiM Yamamoto
Apr 14, 1999·Cell Death and Differentiation·G RaschellàM G Paggi
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Aug 1, 2000·Human Molecular Genetics·A MilolozaM Hengstschläger
Dec 18, 2001·Genes, Chromosomes & Cancer·S A VieiraJ V Melo
Feb 13, 2002·Cell Death and Differentiation·M GruschG Krupitza
Jul 9, 2002·Oncogene·Marion KubistaMarkus Hengstschläger
Aug 29, 2002·The Journal of Biological Chemistry·Masakatsu KobayashiKazuaki Yoshikawa
Sep 3, 2002·Journal of Biochemistry·Hideto Hoshino, Kazuhiko Igarashi
Jan 4, 2003·The Journal of Biological Chemistry·Tomomi KitamuroShigeki Shibahara
Jan 9, 2004·Cell Cycle·Rati FotedarArun Fotedar
Jun 4, 2004·The EMBO Journal·Hiroshi SuzukiKazuhiko Igarashi
Sep 10, 2004·The Journal of Biological Chemistry·Margit Rosner, Markus Hengstschläger
Sep 21, 2004·Oncogene·Lea Harrington

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Citations

Nov 10, 2007·Biochemical and Biophysical Research Communications·Sun Woo HongDong-Ki Lee
Jul 9, 2016·Journal of Cellular Biochemistry·Veronica CartocciValentina Pallottini
May 13, 2010·Journal of Experimental & Clinical Cancer Research : CR·Petr ChlapekRenata Veselska

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