Bacillomycin D-C16 triggers apoptosis of gastric cancer cells through the PI3K/Akt and FoxO3a signaling pathways

Anti-cancer Drugs
Fuxing LinZhaoxin Lu

Abstract

Bacillomycin D can inhibit the growth of Aspergillus ochraceus in food samples. In addition, it can induce apoptosis in and inhibit the proliferation of cancer cells, although the details of this mechanism are unknown. In this study, we separated bacillomycin D-C14, D-C15, D-C16 monomers from the Bacillus subtilis strain fmbJ. The bacillomycin D monomers containing longer fatty acid chains better induced apoptosis in Bgc-823, Sgc-7901, and Hgc-27 gastric cancer cells. The Bgc-823 cell line was the most sensitive. Acridine orange-ethidium bromide staining indicated that bacillomycin D-C16-induced Bgc-823 cell death by triggering apoptosis, characterized by membrane blebbing, cellular shrinkage, and DNA fragmentation. Flow cytometric analysis showed a bacillomycin D-C16 dose-dependent trigger of Bgc-823 apoptosis. Bacillomycin D-C16-induced the mitochondrial pathway, as indicated by a reduced Bcl-2/Bax expression ratio, enhanced cytochrome C release, and higher levels of cleaved caspase-3. Furthermore, bacillomycin D-C16 effectively repressed phosphorylation of the serine-threonine protein kinase Akt at Ser-473 and increased the levels of the FoxO3a protein. The combination of the PI3K/Akt-inhibitor BEZ235 with bacillomycin D-C16...Continue Reading

References

Feb 28, 1998·The EMBO Journal·L O'ConnorD C Huang
Jul 21, 2004·The Journal of Biological Chemistry·Jianhua FengBrian A Hemmings
Dec 24, 2005·The Journal of Biological Chemistry·Yasuko YamamuraYoshiaki Ito
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Rebecca C TaylorSeamus J Martin
Jan 2, 2008·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Jaclyn LoPiccoloPhillip A Dennis
May 29, 2009·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·J-L QuY-P Liu
Feb 18, 2010·Molecular Cell·Jerry E ChipukDouglas R Green
Jul 6, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hsieh-Hsun HoChau-Jong Wang
Jan 6, 2011·FEMS Microbiology Letters·Olfa TabbeneFerid Limam
Oct 1, 2011·Cell Death and Differentiation·T KaufmannP J Jost
Nov 15, 2011·Cell·Yaron Fuchs, Hermann Steller
Sep 23, 2014·Drug Discovery Today·Goutam DeyMahitosh Mandal
Feb 28, 2015·Brain : a Journal of Neurology·Laura A JansenWilliam B Dobyns
Mar 13, 2015·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Takanobu YamadaHeike I Grabsch

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Methods Mentioned

BETA
protein assay
NMR
ELISA
flow cytometry
electrophoresis
PCR
Protein

Software Mentioned

GelAnalyzer
SPSS

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