PMID: 2106512Mar 1, 1990Paper

Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene

Journal of Bacteriology
A SlomaJ Pero

Abstract

We have purified a minor extracellular serine protease from Bacillus subtilis. Characterization of this enzyme indicated that it was most likely the previously reported enzyme bacillopeptidase F. The amino-terminal sequence of the purified protein was determined, and a "guess-mer" oligonucleotide hybridization probe was constructed on the basis of that sequence. This probe was used to identify and clone the structural gene (bpr) for bacillopeptidase F. The deduced amino acid sequence for the mature protein (496 amino acids) was preceded by a putative signal sequence of 30 residues and a putative propeptide region of 164 amino acids. The bpr gene mapped near pyrD on the chromosome and was not required for growth or sporulation.

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Citations

Nov 1, 2005·Journal of Pharmacological Sciences·Kazunobu OmuraShogo Tokudome
Oct 27, 2015·Applied and Environmental Microbiology·Dongheng MengBing Tang
May 29, 2018·Molecular Microbiology·Elliot ErskineCait E MacPhee
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Sep 18, 2004·Journal of Bacteriology·Chi Hye ParkSi Myung Byun
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
Sep 1, 1991·Research in Microbiology·X S HeR H Doi
Jul 1, 1991·Journal of Biotechnology·J VehmaanperäJ Hofemeister
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva
Feb 15, 2007·Journal of Bioscience and Bioengineering·Takeko KodamaJunichi Sekiguchi
Sep 22, 2007·Journal of Bioscience and Bioengineering·Takeko KodamaJunichi Sekiguchi
Aug 20, 2021·FEMS Microbiology Reviews·Colin R Harwood, Yoshimi Kikuchi

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