Bacillus amyloliquefaciens strain 32a as a source of lipopeptides for biocontrol of Agrobacterium tumefaciens strains

Journal of Applied Microbiology
D Ben AbdallahS Tounsi

Abstract

A Bacillus amyloliquefaciens strain, designated 32a, was used to identify new compounds active against Agrobacterium tumefaciens and to evaluate their efficiency to control crown gall on carrot discs. Based on PCR-assays, four gene clusters were shown to direct the synthesis of the cyclic lipopeptides surfactin, iturin A, bacillomycin D and fengycin. Mass spectrometry analysis of culture supernatant led to the identification of these secondary metabolites, except bacillomycin, with heterogeneous mixture of homologues. Antimicrobial assays using lipopeptides-enriched extract showed a strong inhibitory activity against several bacterial and fungal strains, including Ag. tumefaciens. Biological control assays on carrot discs using both 32a spores and extract resulted in significant protection against crown gall disease, similar to that provided by the reference antagonistic strain Agrobacterium rhizogenes K1026. In contrast to all active compounds against Ag. tumefaciens that are of proteinaceous nature, this work enables for the first time to correlate the strong protective effect of B. amyloliquefaciens strain 32a towards crown gall disease with the production of a mixture of lipopeptides. The findings could be useful for grower...Continue Reading

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Citations

May 4, 2016·Biotechnology & Genetic Engineering Reviews·Vivek Rangarajan, Kim G Clarke
Oct 14, 2017·Genome Announcements·Michelle M GerstAhmed E Yousef
Oct 31, 2018·Environmental Science and Pollution Research International·Dorra Ben AbdallahOlfa Frikha-Gargouri
Mar 3, 2019·Applied and Environmental Microbiology·Qian LiHongqing Wang
Feb 28, 2020·International Journal of Environmental Research and Public Health·Deepti MalviyaG P Brahmaprakash
May 18, 2020·Environmental Science and Pollution Research International·Dorra Ben AbdallahOlfa Frikha-Gargouri
Oct 25, 2020·Applied and Environmental Microbiology·Thao D TranJeffery A McGarvey

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