Bacillus amyloliquefaciens TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery

Colloids and Surfaces. B, Biointerfaces
Vanessa Marques AlvarezLucy Seldin

Abstract

A screening for biosurfactant-producing bacteria was conducted with 217 strains that were isolated from environmental samples contaminated with crude oil and/or petroleum derivatives. Although 19 promising biosurfactant producers were detected, strain TSBSO 3.8, which was identified by molecular methods as Bacillus amyloliquefaciens, drew attention for its production of a high-activity compound that presented an emulsification activity of 63% and considerably decreased surface (28.5 mN/m) and interfacial (11.4 mN/m) tensions in Trypticase Soy Broth culture medium. TSBSO 3.8 growth and biosurfactant production were tested under different physical and chemical conditions to evaluate its biotechnological potential. Biosurfactant production occurred between 0.5% and 7% NaCl, at pH values varying from 6 to 9 and temperatures ranging from 28 to 50 °C. Moreover, biosurfactant properties remained the same after autoclaving at 121 °C for 15 min. The biosurfactant was also successful in a test to simulate microbial enhanced oil recovery (MEOR). Mass spectrometry analysis showed that the surface active compound was a surfactin, known as a powerful biosurfactant that is commonly produced by Bacillus species. The production of a high-effici...Continue Reading

References

Dec 1, 1996·Journal of Colloid and Interface Science·B Song, J Springer
Jul 16, 2008·Journal of Microbiological Methods·Harish SutharAnuradha Nerurkar
Dec 5, 2014·Saudi Journal of Biological Sciences·Yesurethinam SarafinThavasimuthu Citarasu
Feb 24, 2015·Extremophiles : Life Under Extreme Conditions·Fábio Sérgio Paulino da SilvaMarcos Rogério Tótola

❮ Previous
Next ❯

Citations

Feb 23, 2020·Biotechnology Progress·Vanessa A L RochaDenise M G Freire
Dec 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nawazish AliMuhammad Tayyab Rashid
Mar 20, 2018·International Journal of Biological Macromolecules·Vanessa Marques AlvarezLucy Seldin
Sep 25, 2021·Applied Biochemistry and Biotechnology·Vasiliki KachrimanidouNikolaos Kopsahelis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.