Bacillus anthracis tagO Is Required for Vegetative Growth and Secondary Cell Wall Polysaccharide Synthesis

Journal of Bacteriology
J M LunderbergO Schneewind

Abstract

Bacillus anthracis elaborates a linear secondary cell wall polysaccharide (SCWP) that retains surface (S)-layer and associated proteins via their S-layer homology (SLH) domains. The SCWP is comprised of trisaccharide repeats [→4)-β-ManNAc-(1→4)-β-GlcNAc-(1→6)-α-GlcNAc-(1→] and tethered via acid-labile phosphodiester bonds to peptidoglycan. Earlier work identified UDP-GlcNAc 2-epimerases GneY (BAS5048) and GneZ (BAS5117), which act as catalysts of ManNAc synthesis, as well as a polysaccharide deacetylase (BAS5051), as factors contributing to SCWP synthesis. Here, we show that tagO (BAS5050), which encodes a UDP-N-acetylglucosamine:undecaprenyl-P N-acetylglucosaminyl 1-P transferase, the enzyme that initiates the synthesis of murein linkage units, is required for B. anthracis SCWP synthesis and S-layer assembly. Similar to gneY-gneZ mutants, B. anthracis strains lacking tagO cannot maintain cell shape or support vegetative growth. In contrast, mutations in BAS5051 do not affect B. anthracis cell shape, vegetative growth, SCWP synthesis, or S-layer assembly. These data suggest that TagO-mediated murein linkage unit assembly supports SCWP synthesis and attachment to the peptidoglycan via acid-labile phosphodiester bonds. Further, B...Continue Reading

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Citations

Aug 28, 2016·FEMS Microbiology Reviews·Christina Schäffer, Paul Messner
Nov 1, 2016·Journal of Bacteriology·So-Young OhDominique Missiakas
Sep 24, 2015·Journal of Bacteriology·Megan Liszewski ZillaDominique Missiakas
Jun 18, 2017·Annual Review of Microbiology·Dominique Missiakas, Olaf Schneewind
Oct 9, 2019·International Journal of Molecular Sciences·Fiona F HagerChristina Schäffer
Jan 23, 2021·International Journal of Molecular Sciences·Cordula StefanovićChristina Schäffer
May 29, 2021·Nature Chemical Biology·Svetlana ZamakhaevaNatalia Korotkova

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