PMID: 6413253Sep 19, 1983Paper

Bacillus cereus 569/H beta-lactamase I: cloning in Escherichia coli and signal sequence determination

FEBS Letters
P S MezesJ O Lampen

Abstract

The gene, penPC, for beta-lactamase I of Bacillus cereus 569/H has been cloned and its expression studied in Escherichia coli. The protein product from the in vitro translation of penPC was shown by gel electrophoresis to have an Mr of 36 000 which is larger than the in vivo products found in B. cereus and E. coli. The DNA sequence of the signal region was determined. It revealed that the smallest known mature form present in B. cereus culture fluids is preceded by 45-48 amino acids in pre-beta-lactamase I, considering that there are 3 initiation codons in the same reading frame, one or more of which might be initiating translation. Unlike the Bacillus licheniformis 749/C beta-lactamase, which has a membrane-bound thioether lipoprotein form, the single Cys residue in the B. cereus beta-lactamase I signal sequence is unmodified and a single processed form of the enzyme is present in E. coli cells carrying penPC.

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C N ChangP Model
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Jan 1, 1973·Annual Review of Genetics·G Zubay
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Jul 14, 1967·Journal of Molecular Biology·D DubnauJ Marmur
Sep 1, 1965·The Biochemical Journal·K A Cammack, H E Wade
Apr 1, 1968·Journal of Bacteriology·M G Sargent
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R P Ambler
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Jan 1, 1982·Biophysical Journal·H C WuC Z Giam
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B NielsenJ O Lampen
Aug 1, 1956·Journal of General Microbiology·M R POLLOCK

❮ Previous
Next ❯

Citations

Nov 6, 2010·Applied Microbiology and Biotechnology·Hiroshi KakeshitaKouji Nakamura
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·R H Doi
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
Jan 1, 1989·Biotechnology & Genetic Engineering Reviews·S UdakaH Yamagata
Jul 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·F GuoT Viswanatha
Jul 11, 1984·Nucleic Acids Research·M E Watson
Jan 1, 1986·Annals of the New York Academy of Sciences·D J McConnellP M Sharp
Nov 22, 1996·The Journal of Biological Chemistry·Y AsanoY Kato
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.