Bacillus cereus endospores exhibit a heterogeneous response to heat treatment and low-temperature storage

Food Microbiology
Ultan P Cronin, Martin G Wilkinson

Abstract

Bacillus cereus endospores were challenged by heat treatments simulating typical domestic/industrial cooking regimes and the resulting effects on germination, viability and sub-lethal heat damage determined using differential plate counting on a rich versus selective medium, flow cytometry (FCM), beta-D-glucuronidase (GUD) activity and OD(600) measurement. Additionally, these techniques were used to investigate the effect on endospores of storage in a non-nutrient medium at 4 degrees C for 1 month. Plate counting revealed that heating generated sub-populations of sub-lethally damaged endospores, with the more severe heat treatments generating larger proportions of sub-lethally damaged endospores. These findings were also reflected in FCM analyses, which detected large amounts of heterogeneity among the populations of heat-treated endospores and uncovered differences in the proportions of membrane-damaged endospores and those displaying esterase activity pre- and post-treatment. Plate count data suggested that both the control and heat-treated endospores lost viability during storage, with FCM data indicating that the proportion of membrane-damaged endospores increased and those displaying the esterase activity decreased. The FC...Continue Reading

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Citations

Jul 16, 2013·Applied and Environmental Microbiology·Robyn T Eijlander, Oscar P Kuipers
Aug 9, 2011·Applied and Environmental Microbiology·Jose-Luis Sanchez-SalasPeter Setlow
Mar 13, 2014·International Journal of Food Microbiology·C C J van MelisT Abee
Sep 22, 2009·Early Human Development·Karen Simmer, Ben Hartmann
Dec 6, 2014·Food Microbiology·Kai DengDaniel Paredes-Sabja
Oct 3, 2014·BioMed Research International·Verónica Ambriz-AviñaMario Pedraza-Reyes
Mar 3, 2015·International Journal of Food Microbiology·Alicja K WardaMasja N Nierop Groot
Oct 1, 2016·Applied Microbiology and Biotechnology·Benjamin BuysschaertNico Boon
Oct 30, 2016·Frontiers in Microbiology·Robyn T EijlanderOscar P Kuipers
Jun 3, 2009·Journal of Applied Microbiology·U P Cronin, M G Wilkinson
Jan 1, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cristina García-TimermansNico Boon
Aug 23, 2019·FEMS Microbiology Ecology·Renxing LiangTullis C Onstott
Nov 13, 2018·MSystems·Jingwei CaiAndrew D Patterson
Dec 3, 2008·Journal of Food Protection·Ultan P Cronin, Martin G Wilkinson
Apr 13, 2017·Food Microbiology·Kai DengJ Antonio Torres

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