Bacillus circulans MTCC 7906 aided facile development of bioconjugate nano-silica alkaline protease formulation with superlative dehairing potential.

Environmental Pollution
Nishu JoshiHarmanjit Singh Banga

Abstract

The tannery industries utilize environmentally hazardous chemicals to achieve dehairing of animal hides, which causes enormous waterbed pollution & high TDS load. Alkaline protease enzyme for dehairing can be an effective solution to resolve the environmental problems of the tannery industry waste. However, stable, cost-efficient and eco-benign formulations of alkaline protease need to be developed for commercial applications in the tannery industry. This works aimed at development of a nano-formulation of the enzyme alkaline protease (AKP) as a bioconjugate nano silica-alkaline protease enzyme (BC-SiNP-AKP). This work reports one pot green synthesis of the BC-SiNP-AKP bionanoconjugate complex which included both biotemplating and immobilization of the AKP on to the synthesized silica nanoparticles from cell-free extracts of Bacillus circulans grown in potato peel based medium. Among the cell free crude, acetone concentrated and purified sols of the enzyme AKP, acetone precipitated enzyme sol was found to be best for the biological SiNP synthesis and formation of BC-SiNP-AKP conjugate. The BC-SiNP-AKP had size ranging from 100 to 200 nm with crystalline morphologies varying from spherical, tubular to laminated crystallites. The...Continue Reading

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Feb 28, 2020·International Journal of Biological Macromolecules·Nishu JoshiHarmanjit Singh Banga

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Citations

Jun 26, 2021·Frontiers in Human Neuroscience·Karmele Lopez-de-IpinaJohn Suckling

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