Abstract
The penicillinase of Bacillus licheniformis is shown to be secreted cotranslationally. In extracts it was formed by membrane-associated but not by free polysomes; and after extracellular labeling of cells, followed by completion of the growing chains on polysomes in vitro, labeled penicillinase could be immunoprecipitated. This product contained electrophoretic peaks of Mr 36,000, 33,000, and 29,000, which correspond to previously reported forms of the enzyme. The Mr 36,000 form exhibits moderate hydrophobicity, as expected of a precursor with an NH2-terminal signal sequence for secretion. In addition, part of the Mr 33,000 fraction evidently contains a lipid: it is even more hydrophobic, and [2-3H]glycerol was found to be incorporated into these molecules but not into the other forms of the enzyme. These findings renew the earlier, discarded suggestion that the Mr 33,000 membrane-bound penicillinase in the cells contains lipid. The incorporation of lipid and two different cleavages can evidently all occur during growth of the penicillinase chain. Moreover, the resulting terminal regions are all accessible to extracellular labeling on growing chains. Several additional, unidentified lipoproteins also incorporate lipid during ch...Continue Reading
References
Jan 9, 1979·Biochemistry·W P SmithB D Davis
Nov 1, 1978·FEBS Letters·M SarvasK Simons
Dec 25, 1978·Journal of Molecular Biology·K SimonsA Helenius
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Inouye, J Beckwith
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W P SmithB D Davis
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Yamamoto, J O Lampen
Dec 1, 1975·The Journal of Cell Biology·G Blobel, B Dobberstein
Oct 19, 1976·Biochimica Et Biophysica Acta·P S Aiyappa, J O Lampen
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Shaltiel, Z Er-El
Sep 27, 1972·Nature: New Biology·C MilsteinM B Mathews
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J Modolell, B D Davis
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J O LampenM P Caulfield
Apr 29, 1980·Biochemistry·K IzuiJ O Lampen
Jan 1, 1980·FEBS Letters·J J LinH C Wu
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J S LaiH C Wu
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B NielsenJ O Lampen
Jan 31, 1980·Nature·B D Davis, P C Tai
Oct 1, 1961·Journal of General Microbiology·M R POLLOCK
Citations
Nov 30, 1982·Biochimica Et Biophysica Acta·A I Magee, M J Schlesinger
Sep 1, 1991·Research in Microbiology·H HemiläI Palva
Jan 1, 1982·Biophysical Journal·H C WuC Z Giam
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J S LaiH C Wu
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B NielsenJ O Lampen
Jan 25, 1983·Nucleic Acids Research·M YangD Henner
Jul 1, 2016·Annual Review of Microbiology·Jeremy C HendersonM Stephen Trent
Jun 27, 1983·FEBS Letters·M Hussain, J O Lampen
Mar 1, 1995·Journal of Bacteriology·I C Sutcliffe, R R Russell
Sep 1, 1983·Microbiological Reviews·T J SilhavyS D Emr
Jun 1, 1983·Journal of Bacteriology·D Marty-MazarsB D Davis
Sep 1, 1996·Journal of Bacteriology·C CordesS Bron
Nov 1, 1988·Journal of Bacteriology·S HayashiY Hirota
Apr 1, 1985·Journal of Bacteriology·I UlmanenI Palva
Aug 1, 1983·Journal of Bacteriology·M O Sarvas, I A Palva
Jun 1, 1986·Journal of Bacteriology·H DohertyP Owen
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva