PMID: 7022452Jun 1, 1981Paper

Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride

Proceedings of the National Academy of Sciences of the United States of America
J S LaiH C Wu

Abstract

DNA sequence analysis of the structural gene for Bacillus licheniformis penicillinase has revealed a tetrapeptide sequence of Leu-Ala-Gly-Cys within the NH2-terminal part of the precursor form of penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6). The same tetrapeptide occurs in the signal sequence of the prolipoprotein of Escherichia coli, and the cysteine residue in the tetrapeptide of prolipoprotein is modified to form glyceride-cysteine which becomes the NH2 terminus of Braun's lipoprotein. On the basis of labeling, with [2-3H]glycerol, [3H]palmitate, [35S]methionine, and [35S]sulfuric acid, of an E. coli strain lysogenic for a lambda vector containing the penicillinase gene from B. licheniformis and of immunoprecipitation with rabbit antisera against purified B. licheniformis penicillinase, we conclude that B. licheniformis penicillinase synthesized in E. coli contains covalently linked glyceride and fatty acid. These results strongly suggest the operation of a modification system in E. coli, and presumably in other Gram-negative bacteria, which results in the formation of a glyceride-cysteine residue if the proper peptide sequence is present in the signal sequence of membrane proteins.

References

Dec 25, 1978·Journal of Molecular Biology·K SimonsA Helenius
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Yamamoto, J O Lampen
Nov 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M Inouye, J P Guthrie
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W P SmithB D Davis
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B NielsenJ O Lampen
Oct 1, 1961·Journal of General Microbiology·M R POLLOCK

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Citations

Jan 1, 1985·Molecular & General Genetics : MGG·D CavardC Lazdunski
Nov 30, 1982·Biochimica Et Biophysica Acta·A I Magee, M J Schlesinger
Jan 1, 1982·Biophysical Journal·H C WuC Z Giam
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W P SmithB D Davis
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B NielsenJ O Lampen
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M TokunagaH C Wu
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
May 1, 1995·Molecular Microbiology·K EhlertM F Templin
Aug 1, 1990·Molecular Microbiology·B K PurcellJ D Radolf
May 1, 1991·Molecular Microbiology·V P KontinenM Sarvas
Jan 1, 1983·Journal of Cellular Biochemistry·H C WuC Z Giam
Mar 1, 1995·Journal of Bacteriology·I C Sutcliffe, R R Russell
Sep 1, 1983·Microbiological Reviews·T J SilhavyS D Emr
Jan 1, 1993·Journal of Bacteriology·N GaneshkumarP E Kolenbrander
Nov 1, 1988·Journal of Bacteriology·S HayashiY Hirota
Apr 1, 1990·Infection and Immunity·M E BrandtM V Norgard
Oct 1, 1982·Journal of Bacteriology·J B Nielsen, J O Lampen
Sep 1, 1988·Journal of Bacteriology·G A WeinbergR S Munson

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