Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells

Nature
J BieleckiD A Portnoy

Abstract

Intracellular parasites can be classified into those that reside within a host vacuole and those which grow directly in the host cytoplasm. Members of the latter group, which includes Rickettsia, Shigellae, Trypanosoma cruzi, and Listeria monocytogenes, possess haemolytic activity associated with the ability to enter the host cytoplasm. Therefore mutants of L. monocytogenes lacking a pore-forming haemolysin, listeriolysin O, do not escape from the endosomal compartment and consequently fail to become established in the cytoplasm. To examine the role of listeriolysin O, we cloned the structural gene for the L. monocytogenes haemolysin, hlyA, into an asporogenic mutant of Bacillus subtilis under the control of an IPTG-inducible promoter. After being internalized by the macrophage-like cell line J774, haemolytic B. subtilis disrupted the phagosomal membrane and grew rapidly within the macrophage cytoplasm. These results show that a single gene product is sufficient to convert a common soil bacterium into a parasite that can grow in the cytoplasm of a mammalian cell.

References

Jun 1, 1976·The Journal of Experimental Medicine·N Nogueira, Z Cohn
Mar 15, 1989·Molecular and Biochemical Parasitology·N W Andrews, M B Whitlow
Apr 1, 1988·The Journal of Experimental Medicine·D A PortnoyD J Hinrichs

❮ Previous
Next ❯

Citations

Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I Rosenshine, B B Finlay
Dec 30, 1998·Archives of Pharmacal Research·G M Larson, K D Lee
May 22, 2010·Journal of Clinical Immunology·Jianghong WuEmad S Alnemri
Jan 1, 1994·Molecular Aspects of Medicine·G MenestrinaC Montecucco
Jan 1, 1991·Parasitology Today·N W Andrews, P Webster
Jul 1, 1993·Veterinary Microbiology·N ChanterT J Alexander
Oct 1, 1991·Research in Immunology·J C BaraleC Braun-Breton
Nov 1, 1994·Research in Microbiology·M P ConteL Seganti
Feb 1, 1992·Current Opinion in Immunology·D A Portnoy
Aug 1, 1994·Trends in Microbiology·N W Andrews, D A Portnoy
May 4, 2004·Vaccine·Le H DucSimon M Cutting
Nov 18, 2000·Progress in Lipid Research·D C DrummondJ Leroux
May 1, 1997·Molecular and Biochemical Parasitology·D K HoweL D Sibley
Mar 4, 2000·Advanced Drug Delivery Reviews·C J Provoda, K D Lee
Aug 5, 2000·Research in Microbiology·M R SpinosaM R Oggioni
Feb 1, 1996·Current Opinion in Immunology·L A Allen, A Aderem
Sep 2, 1998·Current Opinion in Immunology·H ShenX Fan
Mar 1, 1995·Trends in Cell Biology·N W Andrews
Mar 1, 1995·Trends in Cell Biology·S C Silverstein
Feb 13, 2001·Trends in Microbiology·G DietrichW Goebel
Aug 6, 2002·Trends in Microbiology·Mary O'Riordan, Daniel A Portnoy
Nov 7, 2002·Trends in Microbiology·José A Vázquez-Boland
Sep 19, 1997·Trends in Microbiology·M F Horta
Jun 22, 2001·Microbes and Infection·J A Vázquez-BolandW Goebel
Jun 18, 2002·Microbes and Infection·Flávia R Almeida-CamposM Fátima Horta
Dec 3, 1999·Microbes and Infection·D H Schmiel, V L Miller
Feb 19, 2000·Current Opinion in Microbiology·W Goebel, M Kuhn
Sep 22, 1998·Nature Biotechnology·C Grillot-CourvalinP Courvalin
Oct 2, 2004·Nature Reviews. Immunology·Eric G Pamer
Jan 17, 2008·Nature Reviews. Microbiology·Kevin Hybiske, Richard S Stephens
Apr 17, 2009·Nature Reviews. Microbiology·Katrina RayChristoph M Tang
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J LibbyF Heffron
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·D LaPentaP P Cleary
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J HessS H Kaufmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.