Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator

Journal of Molecular Biology
B R Belitsky

Abstract

Bacillus subtilis GabR is a member of a poorly characterized but widespread family of chimeric bacterial proteins that have apparent DNA binding and aminotransferase domains. GabR positively regulates expression of the gabTD operon responsible for utilization of gamma-aminobutyric acid (GABA) and represses the divergently transcribed gabR gene. Purified GabR bound specifically to the DNA region overlapping the -35 region of the gabT promoter and the -10 and +1 regions of the gabR promoter. Two 6 bp direct repeats located at the ends of this region appeared to be essential for GabR binding. In transcription reactions in vitro, GabR alone repressed expression from the gabR promoter but activated expression from the gabT promoter only in the presence of GABA and pyridoxal 5'-phosphate, an essential cofactor of aminotransferases. A similar requirement for pyridoxal 5'-phosphate and GABA for GabR-mediated transcription activation was shown in vivo. In vitro this requirement could be partially satisfied with pyridoxamine 5'-phosphate and succinic semialdehyde, the products of a GABA-dependent aminotransferase half-reaction. We hypothesize that the GabR-catalyzed aminotransferase-like reaction between GABA and pyridoxal 5'-phosphate i...Continue Reading

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Citations

Mar 17, 2010·Applied Microbiology and Biotechnology·Ulla HynönenAiri Palva
Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Raji EdayathumangalamDali Liu
Jun 1, 2015·Computational Biology and Chemistry·Teresa MilanoStefano Pascarella
Mar 4, 2015·Biochimica Et Biophysica Acta·Barbara CampaniniAndrea Mozzarelli
Sep 19, 2016·Biochimica Et Biophysica Acta. General Subjects·Davide AmidaniClaudio Rivetti
Oct 22, 2013·Journal of Bacteriology·Adrienne ZaprasisErhard Bremer
Sep 5, 2019·Molecular Microbiology·Laurent BouillautBoris R Belitsky
Apr 8, 2020·Journal of Bacteriology·Tomokazu Ito, Diana M Downs
Nov 1, 2008·The Journal of Biological Chemistry·Catherine TanousIsabelle Martin-Verstraete
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rui WuDali Liu
Jun 20, 2020·Protein Science : a Publication of the Protein Society·Daniel S CatlinDali Liu
Jan 18, 2016·Microbial Genomics·Inna A Suvorova, Dmitry A Rodionov
May 28, 2019·Frontiers in Molecular Biosciences·Björn RichtsFabian M Commichau

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