PMID: 2506524Aug 25, 1989Paper

Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA alkyltransferase

Nucleic Acids Research
F MorohoshiN Munakata

Abstract

We have cloned a Bacillus subtilis DNA fragment that could correct the defect in a constitutive O6-methylguanine-DNA alkyltransferase (Dat1). This fragment also corrected the hypersensitivity of the strain TKJ6951(ada-1 dat-1) to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In the fragment, the gene activity resides in a region of about 850 bp which contains an open reading frame capable of coding for a protein of 165 amino acid residues. The amino acid sequence of this protein exhibits striking similarity to those of E. coli O6-methylguanine-DNA alkyltransferases (Ogt and Ada proteins). We conclude that this is a structural gene for the Dat1 protein, which is distinct from inducible DNA alkyltransferases involved in the adaptive response. The dat-1 mutation was shown to be caused by a structural rearrangement affecting the coding region, and the 0.8 kb transcripts of this gene were detected in dat+ cells but not in dat mutant cells.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P F Schendel, P E Robins
Jan 1, 1988·Annual Review of Biochemistry·T LindahlY Nakabeppu
Feb 1, 1986·Molecular & General Genetics : MGG·F Morohoshi, N Munakata
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G W RebeckL Samson
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B DempleT Lindahl

❮ Previous
Next ❯

Citations

Feb 1, 1986·Molecular & General Genetics : MGG·F Morohoshi, N Munakata
Jan 1, 1996·Journal of Cancer Research and Clinical Oncology·M SekiguchiT Tuzuki
Nov 1, 1990·Mutation Research·G P MargisonP M Potter
Oct 25, 1989·Nucleic Acids Research·P M PotterG P Margison
Sep 25, 1990·Nucleic Acids Research·F MorohoshiN Munakata
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Apr 1, 1992·Molecular Microbiology·L Samson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.