Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes

Proceedings of the National Academy of Sciences of the United States of America
Susan H Fisher, Lewis V Wray

Abstract

The Bacillus subtilis GlnR repressor controls gene expression in response to nitrogen availability. Because all GlnR-regulated genes are expressed constitutively in mutants lacking glutamine synthetase (GS), GS is required for repression by GlnR. Feedback-inhibited GS (FBI-GS) was shown to activate GlnR DNA binding with an in vitro electophoretic mobility shift assay (EMSA). The activation of GlnR DNA binding by GS in these experiments depended on the feedback inhibitor glutamine and did not occur with mutant GS proteins defective in regulating GlnR activity in vivo. Although stable GS-GlnR-DNA ternary complexes were not observed in the EMSA experiments, cross-linking experiments showed that a protein-protein interaction occurs between GlnR and FBI-GS. This interaction was reduced in the absence of the feedback inhibitor glutamine and with mutant GS proteins. Because FBI-GS significantly reduced the dissociation rate of the GlnR-DNA complexes, the stability of these complexes is enhanced by FBI-GS. These results argue that FBI-GS acts as a chaperone that activates GlnR DNA binding through a transient protein-protein interaction that stabilizes GlnR-DNA complexes. GS was shown to control the activity of the B. subtilis nitrogen ...Continue Reading

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Citations

Jul 27, 2010·Journal of Bacteriology·Lewis V Wray, Susan H Fisher
Feb 24, 2009·Journal of Bacteriology·Susan H Fisher, Lewis V Wray
May 19, 2009·Veterinary Microbiology·Youhui SiHuanchun Chen
Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
Mar 27, 2010·FEMS Microbiology Reviews·Johannes AmonAndreas Burkovski
May 8, 2014·FEMS Microbiology Letters·Daniela KasparStefanie Müller-Herbst
Jun 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Di YouBang-Ce Ye
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Aug 25, 2017·BMC Research Notes·Paola RandazzoSandrine Auger
Jul 22, 2020·Journal of Bacteriology·Rajesh BiswasBoris R Belitsky
Nov 1, 2008·The Journal of Biological Chemistry·Catherine TanousIsabelle Martin-Verstraete
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Sep 10, 2015·Microbiology Spectrum·Fabian M Commichau, Jörg Stülke
Jul 30, 2021·Microbiological Research·Abhinav Nair, Saurabh Jyoti Sarma

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