PMID: 2507520Oct 1, 1989Paper

Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation

Journal of Bacteriology
W MulbryP S Lovett

Abstract

The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochre codon and measuring the level of CAT in the suppressor-containing strain. The results obtained are discussed in light of previous observations that ochre mutations at cat leader codons 2 and 3 can be phenotypically suppressed by sup-3, whereas ochre mutations at leader codons 4 and 5 cannot. Translation of the cat leader is essential to inducible expression of cat. Our data support the interpretation that the nature of amino acids 2 through 5 of the leader peptide contributes to determining whether chloramphenicol can stall a ribosome in the leader, which in turn leads to induction of cat expression.

References

Aug 1, 1979·Journal of Bacteriology·D J Henner, W Steinberg
May 17, 1976·European Journal of Biochemistry·R P MelladoM Salas
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E J Duvall, P S Lovett
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Z AlexievaP S Lovett
Mar 1, 1969·Journal of Bacteriology·C P Georgopoulos
Jan 1, 1983·Methods in Enzymology·M J Zoller, M Smith
Mar 1, 1980·Microbiological Reviews·D J Henner, J A Hoch

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Citations

Jun 12, 2013·Annual Review of Biochemistry·Koreaki Ito, Shinobu Chiba
Apr 9, 2016·Genome Announcements·Christopher M Johnson, Alan D Grossman
May 23, 1997·Journal of Molecular Biology·B BeckerJ C Alonso
Jun 1, 1996·Microbiological Reviews·P S Lovett, E J Rogers
Dec 1, 1993·Journal of Bacteriology·P Wei, C R Stewart
Oct 1, 1993·Journal of Bacteriology·D B Garrity, S A Zahler
Jan 1, 1990·Journal of Bacteriology·P S Lovett

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