Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation

Microbiology
Hideaki NanamiyaFujio Kawamura

Abstract

The number of copies of rRNA genes in bacterial genomes differs greatly among bacterial species. It is difficult to determine the functional significance of the heterogeneity of each rRNA operon fully due to the existence of multiple rRNA operons and because the sequence heterogeneity among the rRNA genes is extremely low. To overcome this problem, we sequentially deleted the ten rrn operons of Bacillus subtilis and constructed seven mutant strains that each harboured a single rrn operon (either rrnA, B, D, E, I, J or O) in their genome. The growth rates and sporulation frequencies of these mutants were reduced drastically compared with those of the wild-type strain, and this was probably due to decreased levels of ribosomes in the mutants. Interestingly, the ability to sporulate varied significantly among the mutant strains. These mutants have proved to be invaluable in our initial attempts to reveal the functional significance of the heterogeneity of each rRNA operon.

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Citations

Mar 27, 2012·Molecular Biology and Evolution·Kyungtaek LimIchizo Kobayashi
Jan 22, 2013·Antimicrobial Agents and Chemotherapy·Yukinori TanakaKozo Ochi
Jun 14, 2013·The Journal of General and Applied Microbiology·Genki AkanumaFujio Kawamura
Apr 2, 2017·Applied and Environmental Microbiology·Blaire StevenJohn Dunbar
May 9, 2019·Scientific Reports·Andreas DomröseAnita Loeschcke
Mar 1, 2019·Frontiers in Microbiology·Ala Eddine Cherni, Xavier Perret
Feb 12, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Anne-Gaëlle PlansonMatthieu Jules

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