Bacillus subtilis MutS Modulates RecA-Mediated DNA Strand Exchange Between Divergent DNA Sequences

Frontiers in Microbiology
Begoña CarrascoJuan C Alonso

Abstract

The efficiency of horizontal gene transfer, which contributes to acquisition and spread of antibiotic resistance and pathogenicity traits, depends on nucleotide sequence and different mismatch-repair (MMR) proteins participate in this process. To study how MutL and MutS MMR proteins regulate recombination across species boundaries, we have studied natural chromosomal transformation with DNA up to ∼23% sequence divergence. We show that Bacillus subtilis natural chromosomal transformation decreased logarithmically with increased sequence divergence up to 15% in wild type (wt) cells or in cells lacking MutS2 or mismatch repair proteins (MutL, MutS or both). Beyond 15% sequence divergence, the chromosomal transformation efficiency is ∼100-fold higher in ΔmutS and ΔmutSL than in ΔmutS2 or wt cells. In the first phase of the biphasic curve (up to 15% sequence divergence), RecA-catalyzed DNA strand exchange contributes to the delineation of species, and in the second phase, homology-facilitated illegitimate recombination might aid in the restoration of inactivated genes. To understand how MutS modulates the integration process, we monitored DNA strand exchange reactions using a circular single-stranded DNA and a linear double-stranded...Continue Reading

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Citations

Mar 3, 2020·Frontiers in Microbiology·Duo-Hong ShengYue-Zhong Li
Mar 1, 2020·BMC Microbiology·Hua ZhouXiaoting Hua
Mar 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey J PowerBerenike Maier

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Methods Mentioned

BETA
restriction modification
electrophoresis
AFM
atomic force microscopy

Software Mentioned

css
WSxM
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