PMID: 8460143Mar 15, 1993Paper

Bacillus subtilis sigma B is regulated by a binding protein (RsbW) that blocks its association with core RNA polymerase

Proceedings of the National Academy of Sciences of the United States of America
A K Benson, W G Haldenwang

Abstract

sigma B is a secondary sigma factor of Bacillus subtilis. RNA polymerase containing sigma B transcribes a subset of genes that are expressed after heat shock or the onset of the stationary phase of growth. Three genes (rsbV, rsbW, and rsbX), cotranscribed with the sigma B structural gene (sigB), regulate sigma B-dependent gene expression. RsbW is the primary inhibitor of this system with the other gene products acting upstream of RsbW in the sigma B regulatory pathway. Evidence is now presented that RsbW inhibits sigma B-dependent transcription by binding to sigma B and blocking the formation of a sigma B-containing RNA polymerase holoenzyme. Antibodies specific for either RsbW or sigma B will coprecipitate both proteins from crude cell extracts. This is not due to the presence of both proteins on RNA polymerase. Western blot analysis of B. subtilis extracts that had been fractionated by gel-filtration chromatography revealed a single peak of RsbW that did not coelute with RNA polymerase and two peaks of sigma B protein: one that eluted with RNA polymerase and a second that overlapped the fractions that contained RsbW. Reconstitution experiments were performed in which partially purified sigma B and RsbW were added to core RNA ...Continue Reading

References

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Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Duncan, R Losick

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Citations

Jul 22, 1995·Molecular & General Genetics : MGG·B MaulM Hecker
Sep 17, 1999·International Journal of Food Microbiology·T Abee, J A Wouters
Jul 1, 1994·Current Biology : CB·C StephensL Shapiro
Oct 1, 1993·Bio/technology·V DereticJ C Boucher
Sep 5, 2001·Molecular Microbiology·C W PriceP Youngman
Feb 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jonathan W YoungMichael B Elowitz
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D W MartinV Deretic
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Duncan, R Losick
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·J DeMaioW R Bishai
May 30, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Masatomo MakinoTakashi Kumasaka
Aug 4, 2006·Applied and Environmental Microbiology·Soraya Chaturongakul, Kathryn J Boor
Apr 27, 2002·Applied and Environmental Microbiology·Oddur Vilhelmsson, Karen J Miller
Sep 16, 2008·Journal of Bacteriology·Eun Sook KimKye Joon Lee
Aug 23, 2006·Journal of Bacteriology·Tatiana A GaidenkoChester W Price
Feb 10, 2009·Journal of Bacteriology·Jan Pané-FarréSusanne Engelmann
Nov 28, 2000·Journal of Bacteriology·L A BeckerA K Benson
Sep 7, 2001·Journal of Bacteriology·A PetersohnM Hecker
Sep 25, 2002·Journal of Bacteriology·Olivier DelumeauMichael D Yudkin
Dec 18, 2002·Journal of Bacteriology·Claudia RollenhagenMichael D Yudkin
Dec 3, 2005·Journal of Bacteriology·Shuyu ZhangW G Haldenwang
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Paul D Cotter, Colin Hill

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