Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon

Journal of Bacteriology
Matthew SchauF Marion Hulett

Abstract

During aerobic respiration, Bacillus subtilis utilizes three terminal oxidases, cytochromes aa3, caa3, and bd. Cytochrome bd is encoded by the cydABCD operon. We report here the first identification of a regulator for the cydABCD operon, YdiH. While working with DeltaresDE mutant strains, we identified colonies which contained suppressor mutations (cmp) which bypassed the requirement for ResD for all phenotypes not associated with cytochrome aa3 or caa3. Mapping identified a class of Tn10 insertions which were close to the cmp locus (Tn10-2) and a second class (Tn10-1) which was inserted in cydD, a gene which appears to be essential to the cmp phenotype. Sequencing of the cmp loci from four independent DeltaresDE cmp isolates yielded four loss-of-function alleles of ydiH, a gene encoding a protein with homology to AT-rich DNA-binding proteins. Additionally, we determined that cytochrome bd was aberrantly expressed in the DeltaresDE cmp background. Together these data led to the hypothesis that YdiH serves as a negative regulator of cydABCD expression, a hypothesis supported by both gel-shift and DNase I footprinting analyses. YdiH protected the cydA promoter region at three 22-bp repeats located in the long 5' untranslated regi...Continue Reading

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Citations

May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Yuji YamamotoPhilippe Gaudu
Nov 22, 2011·Antioxidants & Redox Signaling·Emilio BuenoMaria J Delgado
Apr 13, 2010·Journal of Bacteriology·Bindiya KaushalF Marion Hulett
Jan 24, 2006·Journal of Bacteriology·Heike ReentsElisabeth Härtig
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Greg A Somerville, Richard A Proctor
May 20, 2008·Molecular Microbiology·Ellen WangClaes von Wachenfeldt
Jul 16, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovMichael I Verkhovsky
Dec 3, 2014·Research in Microbiology·Laurent BouillautBruno Dupuy
Jun 2, 2010·Molecular Cell·Krystle J McLaughlinClara L Kielkopf
May 17, 2012·PloS One·Michael Baureder, Lars Hederstedt
May 18, 2016·Toxins·Isabelle Martin-VerstraeteBruno Dupuy
Jul 18, 2015·Microbiology Spectrum·Anthony R RichardsonAbraham L Sonenshein
Jul 28, 2021·Journal of Basic Microbiology·Bingbing HouHuizhan Zhang
Aug 14, 2021·Molecular Microbiology·Erin E PriceJeffrey M Boyd

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