Bacillus thuringiensis HD-73 Spores Have Surface-Localized Cry1Ac Toxin: Physiological and Pathogenic Consequences.

Applied and Environmental Microbiology
C Du, K W Nickerson

Abstract

Spores from Cry(sup+) strains of Bacillus thuringiensis bound fluorescein isothiocyanate-labeled antibodies specific for the 65-kDa activated Cry 1Ac toxin, whereas spores from Bacillus cereus and Cry(sup-) strains of B. thuringiensis did not. The Cry(sup+) spores could be activated for germination by alkaline conditions (pH 10.3), whereas Cry(sup-) spores could not. Once the surrounding exosporia had been removed or permeabilized, Cry(sup+) spores were able to bind the toxin receptor(s) from insect gut brush border membrane vesicle preparations, and their germination rates were increased ca. threefold in the presence of brush border membrane vesicles. A model is presented whereby in the soil the Cry toxins on the spore surface are protected by the exosporium while in the gut they are exposed and available for binding to the insect receptors. This model explains why the disulfide-rich C terminus of the cry genes is so highly conserved even though it is removed during the processing of the protoxin to the activated toxin. It also highlights the trade-off resulting from having Cry toxins located on the spore surface, i.e., decreased spore resistance versus enhanced insect pathogenesis.

References

Sep 1, 1976·Applied and Environmental Microbiology·P GerhardtR Scherrer
Jan 1, 1978·Applied and Environmental Microbiology·J H Schesser, L A Bulla
May 1, 1978·Applied and Environmental Microbiology·V M Griego, K D Spence
Oct 1, 1978·Applied and Environmental Microbiology·B J Ang, K W Nickerson
Oct 16, 1978·Biochemical and Biophysical Research Communications·D P StahlyA I Aronson
Jul 1, 1975·Journal of Bacteriology·A I Aronson, P C Fitz-James
Apr 1, 1975·Journal of General Microbiology·H J Somerville, H V Pockett
May 1, 1992·Journal of Bacteriology·P Setlow
Aug 1, 1990·Applied and Environmental Microbiology·T G BenoitA I Aronson
Jun 1, 1989·Microbiological Reviews·H Höfte, H R Whiteley
Mar 1, 1986·Microbiological Reviews·A I AronsonP Dunn
Jan 1, 1970·Journal of Bacteriology·L L MatzP Gerhardt
Jun 1, 1966·Journal of Invertebrate Pathology·G E Cantwell, B A Franklin
Mar 10, 1995·The Journal of Biological Chemistry·R K VadlamudiL A Bulla
Dec 1, 1995·Current Microbiology·W L Nicholson
Sep 1, 1960·Bacteriological Reviews·A M HEIMPEL, T A ANGUS
Dec 1, 1964·Journal of Bacteriology·P GERHARDT, E RIBI
Jun 1, 1987·Applied and Environmental Microbiology·R S TraversC F Reichelderfer
Oct 1, 1989·Applied and Environmental Microbiology·P A Martin, R S Travers

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Citations

Jun 4, 2005·Applied and Environmental Microbiology·Cheng DuKenneth W Nickerson
Feb 3, 2011·Current Microbiology·Anastasia PapalazaridouAfroditi Sivropoulou
Jun 15, 2011·Journal of Bacteriology·Daisuke ImamuraKazuhito Watabe
Apr 17, 2012·Applied and Environmental Microbiology·Lixin DuDafang Huang
Jul 10, 2012·Applied and Environmental Microbiology·Hui YangDafang Huang
Apr 7, 2019·Applied Microbiology and Biotechnology·Jing LvFuping Song
Jul 23, 2003·Environmental Microbiology·G B JensenJ Mahillon
Jun 26, 2009·Cellular and Molecular Life Sciences : CMLS·Junehyung Kim, Wolfgang Schumann
Sep 4, 1999·Journal of Applied Microbiology·S CharltonA Moir
Nov 18, 2003·Annual Review of Genetics·Ruud A de MaagdH Ernest Schnepf
Mar 6, 2003·Journal of Bacteriology·Christopher SteichenCharles L Turnbough
Aug 4, 2009·Applied and Environmental Microbiology·M M AbdoarrahemC Berry
Apr 19, 2012·PLoS Pathogens·Thomas DuboisDidier Lereclus
Nov 9, 2014·Biotechnology Letters·Estibaliz Sansinenea, Aurelio Ortiz
Nov 24, 2007·Annual Review of Microbiology·Adriano O Henriques, Charles P Moran
Jun 15, 2014·Applied Microbiology and Biotechnology·Changmei ZhouFuping Song
Mar 4, 2017·Scientific Reports·Maria Giovanna MarcheLuca Ruiu
Apr 5, 2018·Genome Biology and Evolution·Krzysztof FiedorukIzabela Swiecicka
Jan 28, 2018·Applied and Environmental Microbiology·Xiaomin ChenFuping Song
Mar 10, 2018·Applied Microbiology and Biotechnology·Xin ZhangFuping Song
Aug 16, 2019·Journal of the Science of Food and Agriculture·Karuna RupulaSashidhar Rao Beedu
May 22, 2019·Microbiology Spectrum·Monika Ehling-SchulzTheresa M Koehler
Jun 25, 2015·Microbiology Spectrum·Rachele Isticato, Ezio Ricca
Feb 1, 1997·Applied and Environmental Microbiology·J J Quinlan, P M Foegeding
Jul 1, 1997·Applied and Environmental Microbiology·A S MazzottaT J Montville
May 1, 1997·Applied and Environmental Microbiology·M R Blake, B C Weimer

❮ Previous
Next ❯

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