Bacillus thuringiensis insecticidal Cry1ab toxin does not affect the membrane integrity of the mammalian intestinal epithelial cells: An in vitro study.

In Vitro Cellular & Developmental Biology. Animal
Nobuaki ShimadaHideo Murata

Abstract

The mammalian intestinal epithelium has been found, based on in vivo experiments, to be resistant to insecticidal Cry toxins, which are derived from Bacillus thuringiensis and fatally damage insect midgut cells. Thus, the toxins are commonly used as a genetic resource in insect-resistant transgenic plants for feed. However, Cry toxins bind to the cellular brush border membrane vesicle (BBMV) of mammalian intestinal cells. In this study, we investigated the affinity of Cry1Ab toxin, a lepidopteran-specific Cry1-type toxin, to the cellular BBMV of two mammalian intestinal cells as well as the effect of the toxin on the membrane potential of three mammalian intestinal cells compared to its effects on the silkworm midgut cell. We found that Cry1Ab toxin did bind to the bovine and porcine BBMV, but far more weakly than it did to the silkworm midgut BBMV. Furthermore, although the silkworm midgut cells developed severe membrane potential changes within 1 h following the toxin treatment at a final concentration of 2 mug/ml, no such membraneous changes were observed on the bovine, porcine, and human intestinal cells. The present in vitro results suggest that, although Cry1Ab toxin may bind weakly or nonspecifically to certain BBMV comp...Continue Reading

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Citations

Nov 6, 2015·Journal of Applied Toxicology : JAT·Néstor Rubio-Infante, Leticia Moreno-Fierros
Nov 7, 2006·The Journal of Veterinary Medical Science·Nobuaki ShimadaShigeru Miyazaki
Mar 8, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Angelika BondzioRalf Einspanier
Jun 2, 2015·PloS One·Manoj S NairMichael K Chan
Aug 3, 2016·Journal of Invertebrate Pathology·Juan Luis Jurat-Fuentes, Neil Crickmore
Oct 23, 2016·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Marwa A A Ibrahim, Ebtsam F Okasha
Jan 1, 2017·Food and Agricultural Immunology·Alexander RazaviChristopher King

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