Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration

Chemical Communications : Chem Comm
ShengQi XiangRasmus Linser

Abstract

NMR characterization of many proteins is limited by low expression, hurdles for deuteration, and poor sample homogeneity. We introduce a set of high-dimensionality proton-detected experiments developed for unambiguous resonance assignments of such proteins, which we could successfully apply to a 1 mg amount of non-deuterated Tau paired helical filaments.

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Citations

Aug 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Loren B AndreasGuido Pintacuda
Sep 28, 2016·Angewandte Chemie·João Medeiros-SilvaMarkus Weingarth
Jun 1, 2017·Journal of Biomolecular NMR·Chang LiuShenlin Wang
Aug 10, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hugo FragaPaul Schanda
Dec 14, 2019·Journal of Biomolecular NMR·Ekaterina BurakovaRasmus Linser
Dec 19, 2019·Chembiochem : a European Journal of Chemical Biology·Vlastimil JiraskoAnja Böckmann
Sep 13, 2018·Journal of Biomolecular NMR·Alons LendsBeat H Meier
Nov 16, 2018·Journal of Biomolecular NMR·Alexander KleinRasmus Linser
Nov 30, 2019·Progress in Nuclear Magnetic Resonance Spectroscopy·Amir Goldbourt
Feb 27, 2018·The Journal of Physical Chemistry Letters·Suresh K VasaRasmus Linser
Apr 30, 2019·The Journal of Physical Chemistry. B·Alexander A ShcherbakovMei Hong
May 16, 2018·Accounts of Chemical Research·Suresh K VasaRasmus Linser

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