Backbone cyclic insulin

Journal of Peptide Science : an Official Publication of the European Peptide Society
Asser S AndersenThomas Hoeg-Jensen

Abstract

Backbone cyclic insulin was designed and prepared by reverse proteolysis in partial organic solvent of a single-chain precursor expressed in yeast. The precursor contains two loops to bridge the two chains of native insulin. The cyclisation method uses Achromobacter lyticus protease and should be generally applicable to proteins with C-terminal lysine and proximal N-terminal. The presence of the ring-closing bond and the native insulin disulfide patterns were documented by LC-MS peptide maps. The cyclic insulin was shown to be inert towards degradation by CPY, but was somewhat labile towards chymotrypsin. Intravenous administration of the cyclic insulin to Wistar rats showed the compounds to be equipotent to HI despite much lower insulin receptor affinity.

References

Mar 1, 1990·Journal of General Microbiology·C VerduynJ P van Dijken
Oct 1, 1990·Journal of Molecular Recognition : JMR·K Morihara
May 15, 1969·The New England Journal of Medicine·D F Steiner
Oct 13, 2000·Applied Microbiology and Biotechnology·T Kjeldsen
May 16, 2001·Journal of Molecular Biology·J A CamareroT W Muir
Mar 15, 2002·Trends in Biochemical Sciences·Manuela Trabi, David J Craik
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Jan Grünewald, Mohamed A Marahiel
Sep 9, 2006·Journal of Dairy Science·A C SørensenP Berg
Mar 12, 2008·The Journal of Biological Chemistry·Qing-xin HuaMichael A Weiss
Apr 11, 2009·The Journal of Biological Chemistry·John M AntosHidde L Ploegh

❮ Previous
Next ❯

Citations

Feb 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Maximilian W PoppHidde L Ploegh
Mar 19, 2016·Nature Reviews. Drug Discovery·Alexander N ZaykovRichard D DiMarchi
Mar 21, 2020·Chemistry : a European Journal·Mads ØstergaardKnud J Jensen
Aug 18, 2020·ACS Omega·Thomas KjeldsenThomas Hoeg-Jensen
May 4, 2011·Angewandte Chemie·Maximilian Wei-Lin Popp, Hidde L Ploegh
Jun 1, 2011·Chemistry : a European Journal·Henrik K MunchKnud J Jensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Protein Expression and Purification
Thomas Kjeldsen, Annette Frost Pettersson
Journal of Peptide Science : an Official Publication of the European Peptide Society
Regina TugyiFerenc Hudecz
© 2021 Meta ULC. All rights reserved