Backbone Hydrogen Bond Energies in Membrane Proteins Are Insensitive to Large Changes in Local Water Concentration.

Journal of the American Chemical Society
Henry J LessenKaren G Fleming

Abstract

A hallmark feature of biological lipid bilayer structure is a depth-dependent polarity gradient largely resulting from the change in water concentration over the angstrom length scale. This gradient is particularly steep as it crosses the membrane interfacial regions where the water concentration drops at least a million-fold along the direction of the bilayer normal. Although local water content is often assumed to be a major determinant of membrane protein stability, the effect of the water-induced polarity gradient upon backbone hydrogen bond strength has not been systematically investigated. We addressed this question by measuring the free energy change for a number of backbone hydrogen bonds in the transmembrane protein OmpW. These values were obtained at 33 backbone amides from hydrogen/deuterium fractionation factors by nuclear magnetic resonance spectroscopy. We surprisingly found that OmpW backbone hydrogen bond energies do not vary over a wide range of water concentrations that are characteristic of the solvation environment in the bilayer interfacial region. We validated the interpretation of our results by determining the hydrodynamic and solvation properties of our OmpW-micelle complex using analytical ultracentrif...Continue Reading

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Citations

Jun 6, 2020·The Journal of Biological Chemistry·Jim E HorneSheena E Radford
Apr 4, 2021·The Journal of Membrane Biology·Sara Capponi
Mar 30, 2021·Biophysical Journal·Yi ZhuangRigoberto Hernandez
May 12, 2021·Current Opinion in Structural Biology·Dagan C Marx, Karen G Fleming
Jan 9, 2021·Journal of the American Chemical Society·Dagan C Marx, Karen G Fleming

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