Background migration of USPIO/MLs is a major drawback for in situ labeling of endogenous neural progenitor cells

Contrast Media & Molecular Imaging
R VreysA Van der Linden

Abstract

MR-labeling of endogenous neural progenitor cells (NPCs) to follow up cellular migration with in vivo magnetic resonance imaging (MRI) is a very promising tool in the rapidly growing field of cellular imaging. To date, most of the in situ labeling work has been performed using micron-sized iron oxide particles. In this work magnetoliposomes (MLs), i.e. ultrasmall superparamagnetic iron oxide cores (USPIOs), each individually coated by a phospholipid bilayer, were used as the MR contrast agent. One of the main advantages of MLs is that the phospholipid bilayer allows easy modification of the surface, which creates the opportunity to construct a wide range of MLs optimized for specific biomedical applications. We have investigated the ability of MLs to label endogenous NPCs after direct injection into the adult mouse brain. Whereas MRI revealed contrast relocation towards the olfactory bulb, our data strongly imply that this relocation is independent of the migration of endogenous NPCs but represents background migration of MLs along a white matter tract. Our findings suggest that the small size of USPIOs/MLs intrinsically limits their potential for in situ labeling of NPCs.

References

May 20, 1994·Science·C Lois, A Alvarez-Buylla
Mar 17, 1999·Journal of Magnetic Resonance Imaging : JMRI·J W BulteJ A Frank
Apr 3, 2001·Nature Reviews. Neuroscience·A Alvarez-BuyllaA D Tramontin
Aug 23, 2001·Neuroscience Letters·T KatoT Moriizumi
Oct 19, 2005·Brain Research. Brain Research Reviews·Peter C BrunjesElizabeth A Meyer
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert G Thorne, Charles Nicholson
Aug 11, 2007·The Journal of Physiology·Mathias HoehnUwe Himmelreich
Oct 19, 2007·Chembiochem : a European Journal of Chemical Biology·Stefaan J H SoenenMarcel De Cuyper
Feb 26, 2008·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Volker MailänderHubert Schrezenmeier
Dec 17, 2008·Contrast Media & Molecular Imaging·Sébastien BoutryRobert N Muller
Apr 18, 2009·Biomaterials·Stefaan J H SoenenMarcel De Cuyper
Sep 8, 2009·Nature Nanotechnology·Iseult LynchKenneth A Dawson

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Citations

Jan 18, 2013·Therapeutic Delivery·Aristides BakandritsosDimitrios G Fatouros
Mar 1, 2014·Tissue Engineering. Part C, Methods·Ashley B AllenRobert E Guldberg
Mar 1, 2012·Translational Stroke Research·Pedro Ramos-Cabrer, Mathias Hoehn
Aug 31, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Greetje Vande VeldeAnnemie van der Linden
Jan 29, 2019·Frontiers in Neuroscience·Christiane L MallettErik M Shapiro
Dec 2, 2020·International Journal of Molecular Sciences·Marina Y KhodanovichAnna V Naumova

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