Mar 20, 2020

BacStalk: a comprehensive and interactive image analysis software tool for bacterial cell biology

Molecular Microbiology
Raimo HartmannKnut Drescher

Abstract

Prokaryotic cells display striking subcellular organization. Studies of the underlying mechanisms in different species have greatly enhanced our understanding of the morphological and physiological adaptation of bacteria to different environmental niches. The image analysis software tool BacStalk is designed to extract comprehensive quantitative information from images of morphologically complex bacteria with stalks, flagella, or other appendages. The resulting data can be visualized in interactive demographs, kymographs, cell lineage plots, and scatter plots to enable fast and thorough data analysis and representation. Notably, BacStalk can generate demographs and kymographs that display fluorescence signals within the two-dimensional cellular outlines, to accurately represent their subcellular location. Beyond organisms with visible appendages, BacStalk is also suitable for established, non-stalked model organisms with common or uncommon cell shapes. BacStalk therefore contributes to the advancement of prokaryotic cell biology and physiology, as it widens the spectrum of easily accessible model organisms and enables highly intuitive and interactive data analysis and visualization.

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Mentioned in this Paper

Analysis
Subcellular Space
Physiological Aspects
Study
Location
Adaptation, Physiological
Cellular Component Organization
Mycobacterium tuberculosis complex
Software Tools
Cytology

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