Bacteria detection based on its blockage effect on silicon nanopore array

Biosensors & Bioelectronics
Yanyan TangJianmin Wu

Abstract

Bacteria detection plays an important role in the guarantee of food and water safety. This work proposed a new sensing strategy for the rapid detection of bacteria based on its blockage effect on nanopore array, which was prepared from electrochemically etched silicon. With the assistance of microfluidic technology, the nanopore array attached with Escherichia coli antibody can selectively and rapidly capture E. coli bacteria, resulting in the decrease of pore accessibility. The signal of pore blockage can be measured by in-direct Fourier Transformed Reflectometric Interference Spectroscopy (FT-RIS). The pore blockage signal has a linear relationship with the logarithm of bacterial density in aqueous sample within the range from 10(3) to 10(7)cfuml(-1). Due to the specific interaction between the antibody and target bacteria, only the E. coli sample displayed significant pore blockage effect, whereas the non-target bacteria, Nox and P17, almost did not show any pore blockage effect. The strategy established in this work might be pervasively applied in the rapid detection of target bacteria and cell in a label-free manner.

References

Jul 18, 2002·Accounts of Chemical Research·J Cooper McDonald, George M Whitesides
Oct 16, 2002·Accounts of Chemical Research·David W Deamer, Daniel Branton
Sep 14, 2012·Small·Jiandong Feng, Jianmin Wu

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Citations

Dec 17, 2016·Biosensors & Bioelectronics· KhoerunnisaSung Young Park
Sep 30, 2017·Chemical Society Reviews·Rasel DasSeeram Ramakrishna
Mar 27, 2020·Frontiers in Chemistry·Gayathri RajeevNicolas H Voelcker
Jan 28, 2021·Biosensors·Roselien VercauterenLaurent A Francis
Jan 1, 2021·Talanta·Shiva AshooriFarshid Raissi
Sep 30, 2020·ACS Applied Materials & Interfaces·Arushi GuptaAmit L Sharma
Nov 10, 2018·Analytical Chemistry·Sofia Arshavsky-GrahamSharon Weiss

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