Bacteria levels in components prepared from deliberately inoculated whole blood held for 8 or 24 hours at 20 to 24 degrees C

Transfusion
S J WagnerG Moroff

Abstract

An increase from 8 to 24 hours in the time that units of whole blood can be held at room temperature after phlebotomy would give blood centers more flexibility in component manufacturing and might allow receipt of many infectious disease test results prior to component preparation. However, the potential for bacterial growth during prolonged holding periods requires further study. In the Phase I study, 2-unit pools of ABO-identical whole blood were deliberately inoculated on Day 0 with Staphylococcus aureus or Pseudomonas fluorescens. They were then divided in half and stored at 20 to 24 degrees C. Red cells (RBCs) with additive solution, platelet concentrates (PCs), and frozen plasma were prepared after 8 and 24 hours. Bacteria levels in PCs and RBCs were monitored on Day 1; bacteria levels were measured in plasma after thawing. In the Phase II study, the same basic design as in Phase I was used, except that 10 bacterial species were studied, lower inocula were used, and RBCs prepared after a 24-hour room-temperature whole-blood hold were white cell-reduced by filtration. Bacterial growth was monitored during 42-day storage of RBCs (1 - 6 degrees C) and 5-day storage of PCs (20 - 24 degrees C) and after thawing of frozen plasm...Continue Reading

Citations

Mar 24, 2000·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·P MorelP Hervé
Nov 30, 2014·Transfusion Medicine Reviews·Pieter F van der Meer, Dirk de Korte

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