Bacterial acetone carboxylase is a manganese-dependent metalloenzyme.

The Journal of Biological Chemistry
Jeffrey M BoydScott A Ensign

Abstract

Bacterial acetone carboxylase catalyzes the ATP-dependent carboxylation of acetone to acetoacetate with the concomitant production of AMP and two inorganic phosphates. The importance of manganese in Rhodobacter capsulatus acetone carboxylase has been established through a combination of physiological, biochemical, and spectroscopic studies. Depletion of manganese from the R. capsulatus growth medium resulted in inhibition of acetone-dependent but not malate-dependent cell growth. Under normal growth conditions (0.5 microm Mn2+ in medium), growth with acetone as the carbon source resulted in a 4-fold increase in intracellular protein-bound manganese over malate-grown cells and the appearance of a Mn2+ EPR signal centered at g = 2 that was absent in malate-grown cells. Acetone carboxylase purified from cells grown with 50 microm Mn2+ had a 1.6-fold higher specific activity and 1.9-fold higher manganese content than cells grown with 0.5 microm Mn2+, consistently yielding a stoichiometry of 1.9 manganese/alpha2beta2gamma2 multimer, or 0.95 manganese/alphabetagamma protomer. Manganese in acetone carboxylase was tightly bound and not removed upon dialysis against various metal ion chelators. The addition of acetone to malate-grown ce...Continue Reading

References

Jan 1, 1990·Archives of Microbiology·K F HillH W Taber
Jan 1, 1990·Archives of Microbiology·H PlatenB Schink
Jan 1, 1989·Annual Review of Biochemistry·J R Knowles
Jan 1, 1987·Archives of Microbiology·H Platen, B Schink
Jul 1, 1969·Journal of Bacteriology·J R Vestal, J J Perry
Mar 1, 1996·Journal of Bacteriology·J R Allen, S A Ensign
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M K Sluis, S A Ensign
Apr 18, 1998·Archives of Microbiology·S A EnsignM K Sluis
Jul 29, 1999·Biochemical Pharmacology·F Y BondocJ Y Hong
Dec 2, 1999·Medical Hypotheses·M P Kalapos
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Oct 25, 2001·The Journal of Biological Chemistry·Brenda S KellyOwen W Griffith
Feb 28, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mei M Whittaker, James W Whittaker
May 1, 1963·Journal of Bacteriology·H B LUKINS, J W FOSTER
Jan 30, 2004·Acta Crystallographica. Section D, Biological Crystallography·Boguslaw NocekJohn W Peters

❮ Previous
Next ❯

Citations

May 29, 2013·Extremophiles : Life Under Extreme Conditions·Ralf SalzerBeate Averhoff
Jun 8, 2011·Metallomics : Integrated Biometal Science·Güneş BenderStephen W Ragsdale
Aug 16, 2011·Applied and Environmental Microbiology·Carlos Henrique DulliusBernhard Schink
Oct 18, 2011·Applied and Environmental Microbiology·Tobias J Erb
Apr 12, 2012·Applied and Environmental Microbiology·Caroline RosierRuddy Wattiez
Oct 6, 2005·Applied and Environmental Microbiology·David A Wampler, Scott A Ensign
Jan 6, 2010·Journal of Bacteriology·Björn JobstJohann Heider
Dec 5, 2006·Journal of Bacteriology·Robert P Hausinger
Aug 6, 2013·Applied and Environmental Microbiology·Olga B Gutiérrez AcostaBernhard Schink
Jun 5, 2020·The Biochemical Journal·Florence MusJohn W Peters
Aug 5, 2017·Scientific Reports·Florence MusJohn W Peters

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.