A surgical procedure allowed the placement of a silicone rubber catheter in the marrow cavity of the tibia of a rabbit and also allowed the introduction of a sclerosing agent (sodium morrhuate) and cells of Staphylococcus aureus. Osteomyelitis developed in 60% of the animals so treated, and the infecting microorganism was recovered from the infected tibias of the animals that developed this disease. All blood cultures taken 24 h after the infection were negative for S. aureus. Radiological findings consisted of osteolytic changes, the occurrence of sequestration and periosteal reactions, and sclerosis in the infected bones. Sections of bone prepared for histological examination confirmed the diagnosis of osteomyelitis. Transmission and scanning electron microscopy of samples of bone marrow, bone chips, and the catheters taken from the infected tibiae revealed gram-positive cocci embedded in a very extensive matrix of ruthenium red-staining glycocalyx adhering to the bone and the implanted catheter. It is proposed that this extensive glycocalyx served a protective function for the bacteria and was important in bacterial adherence and thus played an important role in bacterial persistence and the development of osteomyelitis in t...Continue Reading
Morphological stabilization of capsules of group B streptococci, types Ia, Ib, II, and III, with specific antibody.
Localization of the third component of complement on the cell wall of encapsulated Staphylococcus aureus M: implications for the mechanism of resistance to phagocytosis.
Polysaccharide capsule of Escherichia coli: microscope study of its size, structure, and sites of synthesis.
Cryptic peptidoglycan and the antiphagocytic effect of the Staphylococcus aureus capsule: model for the antiphagocytic effect of bacterial cell surface polymers.
Influence of encapsulation on staphylococcal opsonization and phagocytosis by human polymorphonuclear leukocytes.
Mucoid strains of Pseudomonas aeruginosa: the influence of culture medium on the stability of mucus production
Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action
The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red
Use of specific antibody to demonstrate glycocalyx, K99 pili, and the spatial relationships of K99+ enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves.
Experimental foreign body infections in mice challenged with slime-producing Staphylococcus epidermidis.
Immunologic investigations of mucoid strains of Pseudomonas aeruginosa: comparison of susceptibility to opsonic antibody in mucoid and nonmucoid strains
Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis.
A scanning and transmission electron microscopic study of the surfaces of intrauterine contraceptive devices
Factors affecting complement activation by Staphylococcus aureus cell walls, their components, and mutants altered in teichoic acid.
PRODUCTION OF EXPERIMENTAL OSTEOMYELITIS IN RABBITS BY INTRAVENOUS INJECTION OF STAPHYLOCOCCUS AUREUS
Evaluation of four experimental osteomyelitis infection models by using precolonized implants and bacterial suspensions
Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections
A simple infection model using pre-colonized implants to reproduce rat chronic Staphylococcus aureus osteomyelitis and study antibiotic treatment
Development and current use of local antibiotic carriers in spondylodiscitis : Pilot study on reduction of duration of systemic treatment
The growth of compact and diffuse variants of Staphylococcus aureus in bovine mastitic and normal whey
High and low pressure pulsatile lavage of contaminated tibial fractures: an in vitro study of bacterial adherence and bone damage
Guideline for prevention of surgical site infection, 1999. Hospital Infection Control Practices Advisory Committee
Antibiotic-loaded biodegradable bone cement for prophylaxis and treatment of experimental osteomyelitis in rats
Intracellular Staphylococcus aureus and antibiotic resistance: implications for treatment of staphylococcal osteomyelitis
Effect of silver content on the structure and antibacterial activity of silver-doped phosphate-based glasses.
Experimental model of biofilm implant-related osteomyelitis to test combination biomaterials using biofilms as initial inocula
Evaluation of antibacterial activity and cytocompatibility of ciprofloxacin loaded gelatin-hydroxyapatite scaffolds as a local drug delivery system for osteomyelitis treatment
A prospective, randomized clinical trial comparing an antibiotic-impregnated bioabsorbable bone substitute with standard antibiotic-impregnated cement beads in the treatment of chronic osteomyelitis and infected nonunion
Efficacy of moxifloxacin compared to teicoplanin in the treatment of implant-related chronic osteomyelitis in rats
Quantitation and relative distribution of extracellular matrix in Staphylococcus epidermidis biofilm
The sustained release of antibiotic from freeze-dried fibrin-antibiotic compound and efficacies in a rat model of osteomyelitis
A Novel Repressor of the ica Locus Discovered in Clinically Isolated Super-Biofilm-Elaborating Staphylococcus aureus
Interaction of a pyelonephritogenic Escherichia coli strain with the tissue components of the mouse urinary tract
Advances in in vitro and in vivo models for studying the staphylococcal factors involved in implant infections
Efficacy of colistin alone and in various combinations for the treatment of experimental osteomyelitis due to carbapenemase-producing Klebsiella pneumoniae
Vancomycin-laden calcium phosphate-calcium sulfate composite allows bone formation in a rat infection model
Mechanical Properties of the Composite Material consisting of β-TCP and Alginate-Di-Aldehyde-Gelatin Hydrogel and Its Degradation Behavior.
Application of a rat osteomyelitis model to compare in vivo and in vitro the antibiotic efficacy against bacteria with high capacity to form biofilms
CRISPR & Staphylococcus
CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.