DOI: 10.1101/499731Dec 19, 2018Paper

Bacterial analogs of plant piperidine alkaloids mediate microbial interactions in a rhizosphere model system

BioRxiv : the Preprint Server for Biology
Gabriel L LozanoJo Handelsman

Abstract

Plants expend significant resources to select and maintain rhizosphere communities that benefit their growth and protect them from pathogens. A better understanding of assembly and function of rhizosphere microbial communities will provide new avenues for improving crop production. Secretion of antibiotics is one means by which bacteria interact with neighboring microbes and sometimes change community composition. In our analysis of a taxonomically diverse consortium from the soybean rhizosphere, we found that Pseudomonas koreensis selectively inhibits growth of Flavobacterium johnsoniae and other members of the Bacteroidetes grown in soybean root exudate. A genetic screen in P. koreensis identified a previously uncharacterized biosynthetic gene cluster responsible for the inhibitory activity. The metabolites were isolated based on biological activity and were characterized using tandem-mass spectrometry, multidimensional NMR, and Mosher ester analysis, leading to the discovery of a new family of bacterial piperidine alkaloids, koreenceine A-D. Three of these metabolites are analogs of the plant alkaloid γ-coniceine. Comparative analysis of the koreenceine cluster with the γ-coniceine pathway revealed distinct polyketide syntha...Continue Reading

Related Concepts

Analogs & derivatives
Antibiotics
Cocaine
Biological Evolution
Gene Clusters
Pseudomonas
Polyketide synthase
gamma-coniceine
Crops, Agricultural
Plant alkaloid

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