Abstract
Although microarray technology has become more widespread as a discovery tool for bacterial pathogenesis, it remains a method available only to laboratories with access to expensive equipment and costly analysis software. Mycobacterium tuberculosis, the causative agent for tuberculosis (TB), afflicts one-third of the global population, and kills between 2 and 3 million people per year. While the majority of cases of TB occur in developing areas of the world, facilities in these regions may not be able to support microarray analysis. Additionally, a major limitation of microarrays is that only genes on the array are being assayed. With acquired virulence and drug resistance in microbes, a method less dependent on a predetermined list of gene targets is advantageous. We present a method of expression analysis based on bacterial artificial chromosomes (BACs) that can be applied with standard laboratory equipment and free analysis software. This technique, bacterial artificial chromosome fingerprint arrays (BACFA), was developed and utilised to identify expression differences between intracellular strains of M. tuberculosis, one virulent (H37Rv) and one attenuated (H37Ra). Southern blots of restriction-enzyme digested BAC fragments...Continue Reading
References
Apr 29, 1999·Microbiology·S V GordonS T Cole
May 13, 1999·Molecular Microbiology·S V GordonS T Cole
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E Graham, J E Clark-Curtiss
Jun 3, 2000·Nature Biotechnology·A M TalaatS A Johnston
Oct 6, 2000·Analytical Biochemistry·T D SchmittgenM W Reed
Sep 11, 2001·Journal of Medical Microbiology·N LariC Garzelli
Mar 22, 2002·Analytical Biochemistry·Jo VandesompeleFrank Speleman
Apr 4, 2002·Molecular Microbiology·Joanna C BettsKen Duncan
Aug 15, 2002·Microbiology·David L LakeyPeter F Barnes
Oct 16, 2002·Infection and Immunity·Gael FenhallsKen Duncan
Oct 18, 2002·Nucleic Acids Research·Adel M TalaatStephen Albert Johnston
Nov 2, 2002·Molecular Microbiology·Alexander S PymStewart T Cole
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sarah A StanleyJeffery S Cox
Jun 23, 2004·Tuberculosis·Joanna BaconPhilip D Marsh
Jun 23, 2004·Tuberculosis·D G Niranjala MuttucumaruTanya Parish
Jan 5, 2005·Journal of Bacteriology·María Del Carmen MenéndezMaría Jesús García
Mar 27, 2007·Journal of Bacteriology·Adel M TalaatStephen A Johnston
Citations
Mar 2, 2012·Proteome Science·Neelja SinghalDeepa Bisht
Jun 16, 2010·PloS One·Alice H LiRichard W Stokes
Dec 10, 2009·Future Microbiology·Richard W Stokes, Simon J Waddell