Bacterial Associations Across House Fly Life History: Evidence for Transstadial Carriage From Managed Manure

Journal of Insect Science
Klara Zurek, Dana Nayduch

Abstract

House flies (Diptera: Muscidae; Musca domestica L.) associate with microbe-rich substrates throughout life history. Because larvae utilize bacteria as a food source, most taxa present in the larval substrate, e.g., manure, are digested or degraded. However, some species survive and are present as third-instar larvae begin pupation. During metamorphosis, many bacteria are again lost during histolysis of the larval gut and subsequent remodeling to produce the gut of the imago. It has been previously demonstrated that some bacterial species survive metamorphosis, being left behind in the puparium, present on the body surface, or in the gut of the emerged adult. We used a combined culture-molecular approach to identify viable microbes from managed manure residue and a wild population of house fly larvae, pupae, puparia, and adults to assess transstadial carriage. All larval (10/10), pupal (10/10), and puparial (10/10) cultures were positive for bacteria. Several bacterial species that were present in larvae also were present either in pupae or puparia. Four viable bacterial species were detectable in 6 of 10 imagoes reared from manure. Of note is the apparent transstadial carriage of Bacillus sonorensis, which has been associated w...Continue Reading

References

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Nov 19, 2011·FEMS Microbiology Ecology·Arvind K GuptaYogesh S Shouche
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Sep 10, 2014·Journal of Dairy Science·Kimberly P BuehnerAlvaro Garcia

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Citations

Oct 3, 2019·Journal of Medical Entomology·Haim BialeElad Chiel
May 14, 2020·Scientific Reports·Nadieh de JongeJeppe Lund Nielsen
May 1, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Akira FukudaYutaka Tamura
Aug 11, 2020·Frontiers in Microbiology·Ruxin DuanNingxin Wang
Dec 18, 2020·Frontiers in Microbiology·Moritz GoldAlexander Mathys

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Methods Mentioned

BETA
PCR

Software Mentioned

CodonCode Aligner
BLAST ( Basic Local Alignment Search Tool

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