Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root Secretion

Plant Physiology
Francesco PiniP S Poole

Abstract

Plants engineer the rhizosphere to their advantage by secreting various nutrients and secondary metabolites. Coupling transcriptomic and metabolomic analyses of the pea (Pisum sativum) rhizosphere, a suite of bioreporters has been developed in Rhizobium leguminosarum bv viciae strain 3841, and these detect metabolites secreted by roots in space and time. Fourteen bacterial lux fusion bioreporters, specific for sugars, polyols, amino acids, organic acids, or flavonoids, have been validated in vitro and in vivo. Using different bacterial mutants (nodC and nifH), the process of colonization and symbiosis has been analyzed, revealing compounds important in the different steps of the rhizobium-legume association. Dicarboxylates and sucrose are the main carbon sources within the nodules; in ineffective (nifH) nodules, particularly low levels of sucrose were observed, suggesting that plant sanctions affect carbon supply to nodules. In contrast, high myo-inositol levels were observed prior to nodule formation and also in nifH senescent nodules. Amino acid biosensors showed different patterns: a γ-aminobutyrate biosensor was active only inside nodules, whereas the phenylalanine bioreporter showed a high signal also in the rhizosphere. T...Continue Reading

Citations

Jan 31, 2018·Nature Reviews. Microbiology·Philip PooleJason Terpolilli
Nov 25, 2017·Frontiers in Microbiology·Alice CheccucciAlessio Mengoni
Sep 12, 2018·Canadian Journal of Microbiology·George C diCenzoAlessio Mengoni
Apr 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Carmen Sánchez-CañizaresPhilip S Poole
Apr 20, 2018·FEMS Microbiology Reviews·Rachel M Wheatley, Philip S Poole
Apr 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Marcela A Mendoza-SuárezPhilip S Poole
Sep 4, 2018·Frontiers in Plant Science·Chrysi SergakiPatrick Schäfer
Aug 2, 2019·Nature Communications·Barney A GeddesPhilip S Poole
Dec 18, 2019·Nature Microbiology·Min-Hyung RyuChristopher A Voigt
Nov 25, 2020·The ISME Journal·Timothy L HaskettPhilip S Poole
Feb 5, 2021·Environmental Microbiology Reports·Hayley E KnightsPhilip S Poole
Jan 22, 2021·Current Opinion in Plant Biology·Chengsong ZhaoAnna N Stepanova
Feb 27, 2021·Frontiers in Microbiology·Ilenne Del ValleLauren B Stadler
Oct 13, 2020·Trends in Microbiology·Sabrina L RossetSimon K Davy
Jul 26, 2019·Journal of Advanced Research·Akifumi Sugiyama
Mar 20, 2021·Frontiers in Plant Science·Zhiqiang PangYi Cheng
Mar 4, 2021·The New Phytologist·Pankaj TrivediJan E Leach
May 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Annet WesthoekLindsay A Turnbull
Feb 2, 2021·Bioinformatics·Javier Pardo-DiazGesine Reinert
May 25, 2021·Plant & Cell Physiology·Naoki YanagisawaTetsuya Higashiyama
Jun 12, 2021·Bioscience, Biotechnology, and Biochemistry·Akifumi Sugiyama
May 21, 2021·Plant Physiology·Ji-Yun KimMichael M Wudick
Oct 5, 2021·Frontiers in Bioengineering and Biotechnology·Fernanda Miyuki KashiwagiMarcelo Müller-Santos

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