Bacterial colonization and endotoxin contamination of intravenous infusion fluids

The Journal of Hospital Infection
M TrautmannR Marre

Abstract

Bacterial colonization and endotoxin contamination of intravenous infusion fluids and catheter systems were examined in a surgical intensive care unit. Nineteen consecutive patients were randomly assigned to 48 h (N = 8) or 96 h (N = 11) change of infusion systems. Fluid from infusion bottles (51), infusion bottles plus burettes (102) and catheter systems (104) was cultured quantitatively for aerobic and anaerobic bacteria. Swabs (362) were taken from three-way stopcocks before and after the in-line infusion filters. Total and free endotoxin levels in infusion fluids were measured by quantitative chromogenic Limulus assay. The overall rate of bacterial colonization of bottles/burettes was 7.8% at 48 h and 15.7% at 96 h, while colonization rates of catheter fluid were 34.0% and 24.1%, respectively (n.s.). These high rates of colonization, despite regularly reinforced hand disinfection practices, may be explained by the high frequency of manipulations of the catheter systems, during acute interventions in emergency situations. Cell-bound endotoxin was found in 8.8% of the samples, but only 2.5% of the samples contained free endotoxin. The data support the use of in-line infusion filters, with bacterial-retaining property; however...Continue Reading

References

Aug 1, 1979·Annals of Internal Medicine·J D Band, D G Maki
Apr 1, 1991·Chest·S NorwoodV Cortes
Jan 1, 1990·JPEN. Journal of Parenteral and Enteral Nutrition·K HoribeK Okui
Jun 1, 1980·The Journal of Infectious Diseases·D J CleriS J Seligman
Apr 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R C Spencer
Apr 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E González-BarcaF Gudiol

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Citations

Feb 24, 2006·Journal of Perinatal Medicine·Agnes van den HoogenAndré Fleer
Apr 14, 2009·American Journal of Infection Control·Mohammad Motamedifar, Mehrdad Askarian
Aug 5, 2004·American Journal of Infection Control·Matthias TrautmannPhilipp M Lepper
Nov 10, 2016·British Journal of Nursing : BJN·Mauro PittirutiJosie Stone
Sep 17, 2013·The Cochrane Database of Systematic Reviews·Amanda J UllmanClaire M Rickard
Mar 4, 2014·Journal of Infusion Nursing : the Official Publication of the Infusion Nurses Society·Samantha KeoghClaire Rickard
Sep 17, 2003·Anaesthesia and Intensive Care·A TyagiA K Sethi

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