Bacterial communities in different sections of a municipal wastewater treatment plant revealed by 16S rDNA 454 pyrosequencing.

Applied Microbiology and Biotechnology
Lin Ye, Tong Zhang

Abstract

In this study, we successfully demonstrated that 454 pyrosequencing was a powerful approach for investigating the bacterial communities in the activated sludge, digestion sludge, influent, and effluent samples of a full scale wastewater treatment plant treating saline sewage. For each sample, 18,808 effective sequences were selected and utilized to do the bacterial diversity and abundance analysis. In total, 2,455, 794, 1,667, and 1,932 operational taxonomic units were obtained at 3 % distance cutoff in the activated sludge, digestion sludge, influent, and effluent samples, respectively. The corresponding most dominant classes in the four samples are Alphaproteobacteria, Thermotogae, Deltaproteobacteria, and Gammaproteobacteria. About 67 % sequences in the digestion sludge sample were found to be affiliated with the Thermotogales order. Also, these sequences were assigned into a recently proposed genus Kosmotoga by the Ribosomal Database Project classifier. In the effluent sample, we found high abundance of Mycobacterium and Vibrio, which are genera containing pathogenic bacteria. Moreover, in this study, we proposed a method to differentiate the "gene percentage" and "cell percentage" by using Ribosomal RNA Operon Copy Number ...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 3, 1990·Nature·S J GiovannoniK G Field
Jul 1, 1997·Applied and Environmental Microbiology·J SnaidrK H Schleifer
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W B WhitmanW J Wiebe
Jan 11, 2000·Nucleic Acids Research·J A KlappenbachT M Schmidt
Nov 27, 2002·Antonie van Leeuwenhoek·Michael WagnerHolger Daims
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Jan 27, 2007·Genome Research·Daniel H HusonStephan C Schuster
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Nov 29, 2007·The ISME Journal·Luiz F W RoeschEric W Triplett
Oct 25, 2008·Nucleic Acids Research·Zarraz May-Ping LeeThomas M Schmidt
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Aug 1, 2009·International Journal of Systematic and Evolutionary Microbiology·Jonathan L DipippoKenneth M Noll
Mar 20, 2010·Environmental Microbiology·Susan M HuseMitchell L Sogin
Sep 21, 2010·Journal of Bioscience and Bioengineering·Daisuke SasakiYasuo Igarashi
Apr 8, 2011·The ISME Journal·Guus RoeselersJohn F Rawls
May 20, 2011·Molecular Ecology Resources·Travis C Glenn
Jul 26, 2011·Environmental Science & Technology·Lin Ye, Tong Zhang

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Citations

Oct 12, 2013·World Journal of Microbiology & Biotechnology·Tibor BenedekBalázs Kriszt
Mar 20, 2013·Environmental Science and Pollution Research International·Ana Rita Varela, Célia M Manaia
Sep 12, 2013·International Journal of Molecular Sciences·Kadiya CalderónJesús González-López
Nov 26, 2013·Environmental Science and Pollution Research International·Françoise S LucasJean-Marie Mouchel
Sep 26, 2015·International Journal of Environmental Research and Public Health·Mu PengQiuyu Wang
Jan 31, 2016·Antonie van Leeuwenhoek·Milagro Fernández-DelgadoPaula Suárez
Nov 18, 2015·Applied Microbiology and Biotechnology·Xiao-Ming LuJian-Jun Chen
Oct 27, 2015·Current Microbiology·Marija KaevskaPetra Vasickova
Mar 5, 2016·World Journal of Microbiology & Biotechnology·Agnieszka Cydzik-Kwiatkowska, Magdalena Zielińska
Dec 15, 2015·Scientific Reports·Christos GiatsisMarc Verdegem
Aug 20, 2015·Applied Microbiology and Biotechnology·Hongyu WangHuining Zhang
Mar 5, 2016·The Science of the Total Environment·Sara V LundströmD G Joakim Larsson
Apr 12, 2015·Bioresource Technology·Toyokazu MiuraYutaka Nakashimada
May 6, 2016·Frontiers in Microbiology·Kelvin WongMarirosa Molina
Apr 26, 2015·Environmental Science and Pollution Research International·Yu-Mei WeiYi Jin
May 20, 2015·Chemosphere·Kathryn M DochertyKatherine A Walker
Nov 25, 2014·Applied Microbiology and Biotechnology·Jitendra KeshriMaggy N B Momba
Jun 28, 2016·The Science of the Total Environment·Peng WangYan Li
Jun 25, 2016·Environmental Microbiology·Susanne GüntherSusann Müller
Jul 30, 2016·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Woo-Jun SulSung-Cheol Koh

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Datasets Mentioned

BETA
SRA026842.2

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Mothur
ChimeraSlayer
RDP Rarefaction
Greengenes
RDP Complete Linkage
MEGAN
RDP Align
BLAST
RDP
Python

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