Bacterial community dynamics during industrial malting, with an emphasis on lactic acid bacteria

Food Microbiology
A JustéB Lievens


Characterization of the microflora during malting is an essential step towards process management and optimization. Up till now, however, microbial characterization in the malting process has mostly been done using culture-dependent methods, probably leading to biased estimates of microbial diversity. The aim of this study was to characterize the bacterial communities using two culture-independent methods, including Terminal Restriction Fragment Length Polymorphism (T-RFLP) and 454 pyrosequencing, targeting the 16S rRNA gene. Studied samples originated from two harvest years and two malting houses malting the same batch of barley. Besides targeting the entire bacterial community (T-RFLP), emphasis was put on lactic acid bacteria (LAB) (T-RFLP and 454 pyrosequencing). The overall bacterial community richness was limited, but the community structure changed during the process. Zooming in on the LAB community using 454 pyrosequencing revealed a total of 47 species-level operational taxonomic units (OTUs). LAB diversity appeared relatively limited since 88% of the sequences were covered by the same five OTUs (representing members of Weissella, Lactobacillus and Leuconostoc) present in all samples investigated. Fluctuations in the r...Continue Reading


Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 29, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R K Colwell, J A Coddington
Apr 29, 1997·International Journal of Food Microbiology·M E Stiles, W H Holzapfel
Jul 16, 1999·Critical Reviews in Microbiology·I NootsC W Michiels
Jan 28, 2003·Critical Reviews in Microbiology·Frank J CarrNino Maida
Oct 7, 2003·Annual Review of Microbiology·Michael S Rappé, Stephen J Giovannoni
Jun 8, 2006·Journal of Industrial Microbiology & Biotechnology·Arja LaitilaRiikka Juvonen
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Aug 30, 2008·Food Microbiology·Jean-Luc Jany, Georges Barbier
Nov 14, 2008·Nucleic Acids Research·J R ColeJames M Tiedje
Jun 9, 2009·BMC Bioinformatics·Steven W CulmanDaniel H Buckley
Aug 8, 2009·Environmental Microbiology·Jack A GilbertIan Joint
Sep 28, 2010·Antonie van Leeuwenhoek·Arja LaitilaRiikka Juvonen
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight


Apr 9, 2015·Frontiers in Microbiology·Vincenzina FuscoCharles M A P Franz
Oct 7, 2016·Microbial Biotechnology·Francesca De FilippisDanilo Ercolini
Jan 20, 2018·Annual Review of Food Science and Technology·Francesca De FilippisDanilo Ercolini

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