Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant.

Applied and Environmental Microbiology
Romilio T Espejo, Jaime Romero

Abstract

Most copper bioleaching plants operate with a high concentration of sulfate salts caused by the continuous addition of sulfuric acid and the recycling of the leaching solution. Since the bacteria involved in bioleaching have been generally isolated at low sulfate concentrations, the bacterial population in ores leached with the high-sulfate solution (1.25 M) employed in a copper production plant was investigated. The complexity of the original population was assessed by the length pattern of the spacer regions between the 16S and 23S rRNA genes, observed after PCR amplification of the DNA extracted from the leached ore. Six main spacers were distinguished by electrophoretic migration, but they could be further resolved into eight spacers by nucleotide sequence homology. The degree of homology was inferred from the electrophoretic migration of the heteroduplexes formed after hybridization. One of the spacers was indistinguishable from that found in Thiobacillus thiooxidans, four could be related to Thiobacillus ferrooxidans, and three could be related to Leptospirillum ferrooxidans. Only five of the spacers in the original sample could be recovered after culturing in media containing different inorganic energy source. Altogether...Continue Reading

References

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Jan 1, 1997·Applied and Environmental Microbiology·M Vasquez, R T Espejo

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Citations

Mar 4, 2000·Applied and Environmental Microbiology·L HarahucI Suzuki
Sep 22, 2007·Foodborne Pathogens and Disease·Narjol González-EscalonaAngelo DePaola
Jul 27, 2002·Annual Review of Microbiology·Douglas E Rawlings
May 19, 2005·FEMS Microbiology Letters·Narjol González-EscalonaRomilio T Espejo
Sep 3, 2003·FEMS Microbiology Letters·Andrés CasanovaMatilde Jashés M
Oct 11, 2003·Biotechnology Advances·I Suzuki

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