Bacterial Community Structure Dynamics in Meloidogyne incognita-Infected Roots and Its Role in Worm-Microbiome Interactions.

MSphere
T. YergaliyevAmir Szitenberg

Abstract

Plant parasitic nematodes such as Meloidogyne incognita have a complex life cycle, occurring sequentially in various niches of the root and rhizosphere. They are known to form a range of interactions with bacteria and other microorganisms that can affect their densities and virulence. High-throughput sequencing can reveal these interactions in high temporal and geographic resolutions, although thus far we have only scratched the surface. In this study, we have carried out a longitudinal sampling scheme, repeatedly collecting rhizosphere soil, roots, galls, and second-stage juveniles from 20 plants to provide a high-resolution view of bacterial succession in these niches, using 16S rRNA metabarcoding. Our findings indicate that a structured community develops in the root, in which gall communities diverge from root segments lacking a gall, and that this structure is maintained throughout the crop season. We describe the successional process leading toward this structure, which is driven by interactions with the nematode and later by an increase in bacteria often found in hypoxic and anaerobic environments. We present evidence that this structure may play a role in the nematode's chemotaxis toward uninfected root segments. Finall...Continue Reading

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Citations

Jun 25, 2021·Trends in Parasitology·Olivera Topalović, Mette Vestergård

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Datasets Mentioned

BETA
PRJNA614519

Methods Mentioned

BETA
PCR
PCRs
dissection

Software Mentioned

Jupyter
FastTree
Qiime2
q2
MAFFT
BioPython Entrez
PERMANOVA
UniFrac
DADA2
SILVA

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