Bacterial composition of nasal discharge in children based on highly accurate 16S rRNA gene sequencing analysis

Scientific Reports
Kaoru HaroKazumasa Fukuda

Abstract

Nasopharyngeal colonization by bacteria is a prerequisite for progression to respiratory disease and an important source of horizontal spread within communities. We aimed to perform quantitative analysis of the bacterial cells and reveal the microbiota of the nasal discharge in children at the species level based on highly accurate 16S rRNA gene sequencing. This study enrolled 40 pediatric patients with rhinorrhea. The bacterial cells in the nasal discharge were counted by epifluorescence microscopic analysis. The microbiota was analyzed by using the 16S rRNA gene clone library sequencing method. We demonstrated that a high abundance (median 2.2 × 107 cells/mL) of bacteria was contained in the nasal discharge of children. Of the 40 samples, 37 (92.5%) were dominated by OTUs corresponding to Haemophilus aegyptius/influenzae, Moraxella catarrhalis/nonliquefaciens, or Streptococcus pneumoniae. These samples showed higher cell abundance and lower alpha diversity than the remaining three samples in which the other bacteria coexisted. In addition, 12 sequences with low homology to type strains were considered as previously unknown bacterial lineages. In conclusion, the nasal discharge of most young children contains a large amount of...Continue Reading

References

Dec 12, 2001·American Journal of Respiratory and Critical Care Medicine·V BandiS B Greenberg
May 8, 2003·Current Opinion in Infectious Diseases·Timothy F Murphy
Jan 27, 2004·The Pediatric Infectious Disease Journal·Rand S FarjoJanet R Gilsdorf
Mar 5, 2004·The Lancet Infectious Diseases·D BogaertP W M Hermans
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark BlaxterEyualem Abebe
Nov 24, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Nobuyasu YamaguchiMasao Nasu
Mar 24, 2007·The Journal of Hospital Infection·M Van DortF A Sarubbi
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·J Kirk HarrisNorman R Pace
Nov 29, 2008·The New England Journal of Medicine·Sanjay Sethi, Timothy F Murphy
Jan 7, 2010·PloS One·Markus HiltyWilliam O C Cookson
Jan 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dimitri A DiavatopoulosOdilia L Wijburg
Nov 9, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F ResmanK Riesbeck
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Aug 24, 2012·Expert Review of Vaccines·Birgit SimellUNKNOWN Pneumococcal Carriage Group
Feb 18, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A TorresT Welte
Aug 1, 2014·PloS One·Kazumasa FukudaHatsumi Taniguchi
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
May 14, 2016·Human Vaccines & Immunotherapeutics·Marina Cerquetti, Maria Giufrè
Jun 3, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniel E ParkUNKNOWN PERCH Study Group
Jul 1, 2017·American Journal of Respiratory and Critical Care Medicine·Astrid A T M BoschDebby Bogaert
Oct 3, 2017·Briefings in Bioinformatics·Kazutaka KatohKazunori D Yamada
Nov 10, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gregory P DeMuriEllen R Wald
Nov 21, 2017·Current Opinion in Microbiology·Lindsey BomarKatherine P Lemon
Aug 3, 2018·Science Translational Medicine·Joan E NicholsJoaquin Cortiella
Nov 6, 2018·Respiratory Investigation·Kazuhiro YateraHiroshi Mukae
Jun 16, 2019·The Journal of Allergy and Clinical Immunology·Kathryn McCauleyUNKNOWN National Institute of Allergy and Infectious Diseases–sponsored Inner-City Asthma Consortium
Dec 18, 2019·Nature Communications·Yanjiao ZhouAvraham Beigelman
Dec 25, 2019·BMC Pulmonary Medicine·Ken OtsujiMitsumasa Saito

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Citations

Aug 18, 2021·Respiratory Investigation·Kazuhiro YateraHiroshi Mukae

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Datasets Mentioned

BETA
LC558357-LC564861

Methods Mentioned

BETA
bronchoalveolar lavage
PCR
electrophoresis

Software Mentioned

vegan
BellCurve for Excel
RDP Classifier
Classifier
Model Finder
MAFFT
IQ
Seqmatch
RDP
Check Chimera

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