Bacterial CRISPR/Cas DNA endonucleases: A revolutionary technology that could dramatically impact viral research and treatment

Virology
Edward M Kennedy, Bryan R Cullen

Abstract

CRISPR/Cas systems mediate bacterial adaptive immune responses that evolved to protect bacteria from bacteriophage and other horizontally transmitted genetic elements. Several CRISPR/Cas systems exist but the simplest variant, referred to as Type II, has a single effector DNA endonuclease, called Cas9, which is guided to its viral DNA target by two small RNAs, the crRNA and the tracrRNA. Initial efforts to adapt the CRISPR/Cas system for DNA editing in mammalian cells, which focused on the Cas9 protein from Streptococcus pyogenes (Spy), demonstrated that Spy Cas9 can be directed to DNA targets in mammalian cells by tracrRNA:crRNA fusion transcripts called single guide RNAs (sgRNA). Upon binding, Cas9 induces DNA cleavage leading to mutagenesis as a result of error prone non-homologous end joining (NHEJ). Recently, the Spy Cas9 system has been adapted for high throughput screening of genes in human cells for their relevance to a particular phenotype and, more generally, for the targeted inactivation of specific genes, in cell lines and in vivo in a number of model organisms. The latter aim seems likely to be greatly enhanced by the recent development of Cas9 proteins from bacterial species such as Neisseria meningitidis and Stap...Continue Reading

References

Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·T W ChunA S Fauci
Apr 27, 2007·Journal of Virology·Lindsey M Hutt-Fletcher
Feb 18, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Thomas J CradickAnton P McCaffrey
Jul 28, 2010·Genetics·Michelle ChristianDaniel F Voytas
Dec 24, 2010·Nature Biotechnology·Jeffrey C MillerEdward J Rebar
Jan 13, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stéphanie GrosseJulianne Smith
Aug 5, 2011·Nucleic Acids Research·Rimantas SapranauskasVirginijus Siksnys
Apr 13, 2012·Seminars in Cancer Biology·Sai Wah TsaoKwok Wai Lo
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Michael H Malim, Paul D Bieniasz
Jun 22, 2012·Journal of Virology·Joshua T SchifferKeith R Jerome
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Dec 29, 2012·Drug Discovery Today·Sifei Xing, Robert F Siliciano
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lijing LiDavid L DiGiusto
May 15, 2013·Trends in Biotechnology·Thomas GajCarlos F Barbas
Jul 23, 2013·Nature Biotechnology·Patrick D HsuFeng Zhang
Jul 26, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kristie BloomPatrick Arbuthnot
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhonggang HouJames A Thomson
Aug 27, 2013·Scientific Reports·Hirotaka EbinaYoshio Koyanagi
Sep 13, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jieliang ChenZhenghong Yuan
Oct 1, 2013·Nature Methods·Kevin M EsveltGeorge M Church
Dec 10, 2013·Cell Stem Cell·Yuxuan WuJinsong Li
Dec 18, 2013·Science·Tim WangEric S Lander
Dec 18, 2013·Science·Ophir ShalemFeng Zhang
Feb 6, 2014·Molecular Therapy. Nucleic Acids·Martine AubertKeith R Jerome
Mar 19, 2014·Journal of Immunological Methods·Maura Dandri, Marc Lütgehetmann
Apr 23, 2014·Nature Biotechnology·Xuebing WuPhillip A Sharp
Apr 29, 2014·Molecular Cell·Rodolphe Barrangou, Luciano A Marraffini
May 21, 2014·Nature Reviews. Genetics·Melissa A Kotterman, David V Schaffer
Jun 1, 2014·Nucleic Acids Research·Alfred PingoudWolfgang Wende
Jul 22, 2014·Biochemical and Biophysical Research Communications·Shuai ZhenYan Li
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wenhui HuKamel Khalili
Aug 20, 2014·Molecular Therapy. Nucleic Acids·Su-Ru LinPei-Jer Chen

❮ Previous
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Citations

Jan 13, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Morgan L Maeder, Charles A Gersbach
Jul 3, 2015·Evolutionary Applications·Britt Koskella
Jan 12, 2016·Expert Review of Gastroenterology & Hepatology·Maria Grazia Clemente, Pietro Vajro
May 18, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Eleanor M P WilsonShyam Kottilil
May 6, 2016·Annual Review of Chemical and Biomolecular Engineering·Christopher E Nelson, Charles A Gersbach
Jul 9, 2016·Phytopathology·Vincent N FondongSavithramma P Dinesh-Kumar
Sep 13, 2016·Frontiers in Microbiology·Ahmed HadidiMarina Barba
Sep 20, 2016·JCI Insight·Martine AubertKeith R Jerome
Sep 9, 2016·Nature Biotechnology·Rodolphe Barrangou, Jennifer A Doudna
Oct 11, 2016·Current Opinion in Virology·Frank Buchholz, Joachim Hauber
Nov 15, 2016·Scientific Reports·Yi-Ying ChouTom Kirchhausen
Feb 9, 2017·Genome Génome / Conseil National De Recherches Canada·Kumiko Ui-TeiYuko Nakano
Jan 5, 2019·Advanced Pharmaceutical Bulletin·Hadi BayatAzam Rahimpour
Oct 30, 2015·Virologica Sinica·Cheng PengDongliang Yang
Sep 13, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna BellizziHassen S Wollebo
Nov 7, 2019·Current Genetics·Antara BarmanSupriyo Chakraborty
Dec 9, 2015·Proceedings of the National Academy of Sciences of the United States of America·Keith T Gagnon, David R Corey
Sep 14, 2016·Monash Bioethics Review·Carlos Mariscal, Angel Petropanagos
Dec 1, 2017·Current Microbiology·Hadi BayatAzam Rahimpour
Jan 10, 2021·Seminars in Cancer Biology·Imran AnsariSaurabh Singh
Aug 11, 2020·Molecular Therapy. Methods & Clinical Development·Martijn P T ErnstW W M Pim Pijnappel

❮ Previous
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